PUBLICATION
Limb regeneration is impaired in an adult zebrafish model of diabetes mellitus
- Authors
- Olsen, A.S., Sarras, M.P. Jr, and Intine, R.V.
- ID
- ZDB-PUB-101004-2
- Date
- 2010
- Source
- Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 18(5): 532-542 (Journal)
- Registered Authors
- Sarras, Michael P., Jr.
- Keywords
- none
- MeSH Terms
-
- Animal Fins/physiology*
- Animals
- Apoptosis
- Blood Glucose/metabolism
- Cell Proliferation
- Diabetes Mellitus, Experimental/metabolism
- Diabetes Mellitus, Experimental/pathology*
- Disease Models, Animal
- Immunohistochemistry
- Insulin/blood
- Pancreas/metabolism
- Regeneration/physiology*
- Zebrafish
- PubMed
- 20840523 Full text @ Wound Repair Regen.
Citation
Olsen, A.S., Sarras, M.P. Jr, and Intine, R.V. (2010) Limb regeneration is impaired in an adult zebrafish model of diabetes mellitus. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. 18(5):532-542.
Abstract
The zebrafish (Danio rerio) is an established model organism for the study of developmental processes, human disease, and tissue regeneration. We report that limb regeneration is severely impaired in our newly developed adult zebrafish model of type I diabetes mellitus. Intraperitoneal streptozocin injection of adult, wild-type zebrafish results in a sustained hyperglycemic state as determined by elevated fasting blood glucose values and increased glycation of serum protein. Serum insulin levels are also decreased and pancreas immunohistochemisty revealed a decreased amount of insulin signal in hyperglycemic fish. Additionally, the diabetic complications of retinal thinning and glomerular basement membrane thickening (early signs of retinopathy and nephropathy) resulting from the hyperglycemic state were evident in streptozocin-injected fish at 3 weeks. Most significantly, limb regeneration, following caudal fin amputation, is severely impaired in diabetic zebrafish and nonspecific toxic effects outside the pancreas were not found to contribute to impaired limb regeneration. This experimental system using adult zebrafish facilitates a broad spectrum of genetic and molecular approaches to study regeneration in the diabetic background.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping