PUBLICATION
Zebrafish mast cells possess an FcepsilonRI-like receptor and participate in innate and adaptive immune responses
- Authors
- Da'as, S., Teh, E.M., Dobson, J.T., Nasrallah, G.K., McBride, E.R., Wang, H., Neuberg, D.S., Marshall, J.S., Lin, T.J., and Berman, J.N.
- ID
- ZDB-PUB-101004-13
- Date
- 2011
- Source
- Developmental and comparative immunology 35(1): 125-134 (Journal)
- Registered Authors
- Berman, Jason, Da'as, Sahar, Dobson, Tristan
- Keywords
- Zebrafish, Mast cells, Ketotifen, Aeromonas salmonicida, Anaphylaxis, Toll-like receptor
- MeSH Terms
-
- Zebrafish/classification
- Zebrafish/immunology*
- Sequence Alignment
- Receptors, IgE/genetics*
- Receptors, IgE/immunology*
- Molecular Sequence Data
- Humans
- Ketotifen/pharmacology
- Adaptive Immunity*
- Mast Cells/drug effects
- Mast Cells/immunology*
- Amino Acid Sequence
- Histamine H1 Antagonists/pharmacology
- Immunity, Innate*
- Animals
- p-Methoxy-N-methylphenethylamine/pharmacology
- Phylogeny
- PubMed
- 20849876 Full text @ Dev. Comp. Immunol.
Citation
Da'as, S., Teh, E.M., Dobson, J.T., Nasrallah, G.K., McBride, E.R., Wang, H., Neuberg, D.S., Marshall, J.S., Lin, T.J., and Berman, J.N. (2011) Zebrafish mast cells possess an FcepsilonRI-like receptor and participate in innate and adaptive immune responses. Developmental and comparative immunology. 35(1):125-134.
Abstract
We previously identified a zebrafish mast cell (MC) lineage and now aim to determine if these cells function analogously in innate and adaptive immunity like their mammalian counterparts. Intraperitoneal (IP) injection of compound 48/80 or live Aeromonas salmonicida resulted in significant MC degranulation evident histologically and by increased plasma tryptase compared with saline-injected controls (p=0.0006, 0.005, respectively). Pre-treatment with ketotifen abrogated these responses (p=0.0004, 0.005, respectively). Cross-reactivity was observed in zebrafish to anti-human high-affinity IgE receptor gamma (FcεRlγ) and IgE heavy chain-directed antibodies. Whole mount in situ hybridization on 7-day embryos demonstrated co-localization of cpa5, a MC-specific marker, with myd88, a toll-like receptor adaptor, and zebrafish FcεRI subunit homologs. Zebrafish injected IP with matched dinitrophenyl-sensitized mouse (anti-DNP) IgE and DNP-BSA or trinitrophenyl-sensitized mouse (anti-TNP) IgE and TNP-BSA demonstrated increased plasma tryptase compared with mismatched controls (p=0.03, 0.010, respectively). These results confirm functional conservation and validate the zebrafish model as an in vivo screening tool for novel MC modulating agents.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping