ZFIN ID: ZDB-PUB-101004-13
Zebrafish mast cells possess an FcepsilonRI-like receptor and participate in innate and adaptive immune responses
Da'as, S., Teh, E.M., Dobson, J.T., Nasrallah, G.K., McBride, E.R., Wang, H., Neuberg, D.S., Marshall, J.S., Lin, T.J., and Berman, J.N.
Date: 2011
Source: Developmental and comparative immunology   35(1): 125-134 (Journal)
Registered Authors: Berman, Jason, Da'as, Sahar, Dobson, Tristan
Keywords: Zebrafish, Mast cells, Ketotifen, Aeromonas salmonicida, Anaphylaxis, Toll-like receptor
MeSH Terms:
  • Adaptive Immunity*
  • Amino Acid Sequence
  • Animals
  • Histamine H1 Antagonists/pharmacology
  • Humans
  • Immunity, Innate*
  • Ketotifen/pharmacology
  • Mast Cells/drug effects
  • Mast Cells/immunology*
  • Molecular Sequence Data
  • Phylogeny
  • Receptors, IgE/genetics*
  • Receptors, IgE/immunology*
  • Sequence Alignment
  • Zebrafish/classification
  • Zebrafish/immunology*
  • p-Methoxy-N-methylphenethylamine/pharmacology
PubMed: 20849876 Full text @ Dev. Comp. Immunol.
We previously identified a zebrafish mast cell (MC) lineage and now aim to determine if these cells function analogously in innate and adaptive immunity like their mammalian counterparts. Intraperitoneal (IP) injection of compound 48/80 or live Aeromonas salmonicida resulted in significant MC degranulation evident histologically and by increased plasma tryptase compared with saline-injected controls (p=0.0006, 0.005, respectively). Pre-treatment with ketotifen abrogated these responses (p=0.0004, 0.005, respectively). Cross-reactivity was observed in zebrafish to anti-human high-affinity IgE receptor gamma (FcεRlγ) and IgE heavy chain-directed antibodies. Whole mount in situ hybridization on 7-day embryos demonstrated co-localization of cpa5, a MC-specific marker, with myd88, a toll-like receptor adaptor, and zebrafish FcεRI subunit homologs. Zebrafish injected IP with matched dinitrophenyl-sensitized mouse (anti-DNP) IgE and DNP-BSA or trinitrophenyl-sensitized mouse (anti-TNP) IgE and TNP-BSA demonstrated increased plasma tryptase compared with mismatched controls (p=0.03, 0.010, respectively). These results confirm functional conservation and validate the zebrafish model as an in vivo screening tool for novel MC modulating agents.