PUBLICATION

Cranial vasculature in zebrafish forms by angioblast cluster-derived angiogenesis

Authors
Proulx, K., Lu, A., and Sumanas, S.
ID
ZDB-PUB-100929-12
Date
2010
Source
Developmental Biology   348(1): 34-46 (Journal)
Registered Authors
Sumanas, Saulius
Keywords
Zebrafish, Etsrp, Etv2, ER71, Cranial, Vasculogenesis, Angiogenesis, Myelopoiesis, Endothelial, Transgenic
MeSH Terms
  • Neovascularization, Physiologic*
  • Blood Vessels/embryology*
  • Organ Culture Techniques
  • Myeloid Cells/metabolism*
  • Gene Knockdown Techniques
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • In Situ Hybridization
  • Recombinant Fusion Proteins/metabolism
  • Head/blood supply*
  • Head/embryology
  • Gene Expression Regulation, Developmental*
  • Genes, Reporter
  • Endothelial Cells/metabolism*
  • Cell Movement
  • Endocardium/embryology
  • Endocardium/metabolism
  • Animals, Genetically Modified
  • Animals
(all 20)
PubMed
20832394 Full text @ Dev. Biol.
Abstract
Formation of embryonic vasculature involves vasculogenesis as endothelial cells differentiate and aggregate into vascular cords and angiogenesis which includes branching from the existing vessels. In the zebrafish which has emerged as an advantageous model to study vasculogenesis, cranial vasculature is thought to originate by a combination of vasculogenesis and angiogenesis, but how these processes are coordinated is not well understood. To determine how angioblasts assemble into cranial vasculature, we generated an etsrp:GFP transgenic line in which GFP reporter is expressed under the promoter control of an early regulator of vascular and myeloid development, etsrp/etv2. By utilizing time-lapse imaging we show that cranial vessels originate by angiogenesis from angioblast clusters, which themselves form by the mechanism of vasculogenesis. The two major pairs of bilateral clusters include the rostral organizing center (ROC) which gives rise to the most rostral cranial vessels and the midbrain organizing center (MOC) which gives rise to the posterior cranial vessels and to the myeloid and endocardial lineages. In Etsrp knockdown embryos initial cranial vasculogenesis proceeds normally but endothelial and myeloid progenitors fail to initiate differentiation, migration and angiogenesis. Such angioblast cluster-derived angiogenesis is likely to be involved during vasculature formation in other vertebrate systems as well.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ci1TgTransgenic Insertion
    ci2TgTransgenic Insertion
      ci3TgTransgenic Insertion
        ci4TgTransgenic Insertion
          ci5TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            etsrpMO1-etsrpMRPHLNO
            etsrpMO2-etsrpMRPHLNO
            etsrpMO4-etsrpMRPHLNO
            etsrpMO5-etsrpMRPHLNO
            tp53MO4-tp53MRPHLNO
            1 - 5 of 5
            Show
            Fish
            Fish
            ci1Tg (EKW)
            ci2Tg (EKW)
            ci3Tg (EKW)
            ci4Tg (EKW)
            ci5Tg
            1 - 5 of 5
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            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            mCherryEFGmCherry
            1 - 2 of 2
            Show
            Mapping
            No data available
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            Citation
            Proulx, K., Lu, A., and Sumanas, S. (2010) Cranial vasculature in zebrafish forms by angioblast cluster-derived angiogenesis. Developmental Biology. 348(1):34-46.