PUBLICATION
Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma
- Authors
- Koebernick, K., Loeber, J., Arthur, P.K., Tarbashevich, K., and Pieler, T.
- ID
- ZDB-PUB-100910-12
- Date
- 2010
- Source
- Proceedings of the National Academy of Sciences of the United States of America 107(37): 16148-16153 (Journal)
- Registered Authors
- Tarbashevich, Katsiyarina
- Keywords
- germ cell, microRNA, RNA localization, vegetal, development
- MeSH Terms
-
- Animals
- Base Sequence
- Embryo, Nonmammalian/metabolism
- Female
- Gene Expression Regulation, Developmental
- Genes, Reporter
- Germ Cells/metabolism
- MicroRNAs/genetics
- MicroRNAs/metabolism*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism*
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/metabolism*
- Ribonucleoproteins/genetics
- Ribonucleoproteins/metabolism
- Transcription, Genetic
- Xenopus Proteins/genetics*
- Xenopus Proteins/metabolism
- Xenopus laevis/embryology*
- Xenopus laevis/genetics
- Xenopus laevis/metabolism*
- PubMed
- 20805475 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Koebernick, K., Loeber, J., Arthur, P.K., Tarbashevich, K., and Pieler, T. (2010) Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma. Proceedings of the National Academy of Sciences of the United States of America. 107(37):16148-16153.
Abstract
Segregation of the future germ line defines a crucial cell fate decision during animal development. In Xenopus, germ cells are specified by inheritance of vegetally localized maternal determinants, including a group of specific mRNAs. Here, we show that the vegetal localization elements (LE) of Xenopus Dead end (XDE) and of several other germ-line-specific, vegetally localized transcripts mediate germ cell-specific stabilization and somatic clearance of microinjected reporter mRNA in Xenopus embryos. The part of XDE-LE critical for somatic RNA clearance exhibits homology to zebrafish nanos1 and appears to be targeted by Xenopus miR-18 for somatic mRNA clearance. Xenopus Elr-type proteins of the vegetal localization complex can alleviate somatic RNA clearance of microinjected XDE-LE and endogenous XDE mRNA. ElrB1 synergizes with Xenopus Dead end protein in the stabilization of XDE-LE mRNA. Taken together, our findings unveil a functional link of vegetal mRNA localization and the protection of germ-line mRNAs from somatic clearance.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping