PUBLICATION
A Runx1 Intronic Enhancer Marks Hemogenic Endothelial Cells and Hematopoietic Stem Cells
- Authors
- Ng, C.E., Yokomizo, T., Yamashita, N., Cirovic, B., Jin, H., Wen, Z., Ito, Y., and Osato, M.
- ID
- ZDB-PUB-100830-7
- Date
- 2010
- Source
- Stem cells (Dayton, Ohio) 28(10): 1869-1881 (Journal)
- Registered Authors
- Wen, Zilong
- Keywords
- hematopoietic stem cells, hemogenic endothelial cells, enhancer element, AML1, retrovirus integration site
- MeSH Terms
-
- Animals
- Base Sequence
- Cell Line
- Core Binding Factor Alpha 2 Subunit/genetics*
- Enhancer Elements, Genetic/genetics*
- Female
- Flow Cytometry
- Hemangioblasts/metabolism*
- Hematopoietic Stem Cells/metabolism*
- Male
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- PubMed
- 20799333 Full text @ Stem Cells
Citation
Ng, C.E., Yokomizo, T., Yamashita, N., Cirovic, B., Jin, H., Wen, Z., Ito, Y., and Osato, M. (2010) A Runx1 Intronic Enhancer Marks Hemogenic Endothelial Cells and Hematopoietic Stem Cells. Stem cells (Dayton, Ohio). 28(10):1869-1881.
Abstract
Runx1 is essential for the generation of hematopoietic stem cells (HSCs) and is frequently mutated in human leukemias. However, the cis-regulatory mechanisms modulating the Runx1 gene expression remain to be elucidated. Herewith, we report the identification of an intronic Runx1 enhancer, Runx1 +24 mCNE, using a combinatorial in silico approach involving comparative genomics and retroviral integration sites mapping. The Runx1 +24 mCNE was found to possess hematopoietic-specific enhancer activity in both zebrafish and mouse models. Significantly, this enhancer is active specifically in hemogenic endothelial cells (ECs) at sites where the de novo generation of HSCs occurs. The activity of this enhancer is also strictly restricted to HSCs within the hematopoietic compartment of the adult bone marrow. We anticipate that Runx1 +24 mCNE HSC enhancer will serve as a molecular handle for tracing and/or manipulating hemogenic ECs/HSCs behavior in vivo and consequently become an invaluable tool for research on stem cell and cancer biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping