PUBLICATION
Expression of GPR177 (Wntless/Evi/Sprinter), a highly conserved Wnt-transport protein, in rat tissues, zebrafish embryos, and cultured human cells
- Authors
- Jin, J., Morse, M., Frey, C., Petko, J., and Levenson, R.
- ID
- ZDB-PUB-100726-22
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(9): 2426-2434 (Journal)
- Registered Authors
- Levenson, Robert, Petko, Jessica
- Keywords
- GPR177, wntless, zebrafish, rodents, expression, structure/function
- MeSH Terms
-
- Animals
- Brain/cytology
- Brain/metabolism
- Cells, Cultured
- Gene Knockdown Techniques
- HEK293 Cells
- Humans
- Intracellular Signaling Peptides and Proteins/genetics
- Intracellular Signaling Peptides and Proteins/metabolism*
- Mice
- Rats
- Rats, Sprague-Dawley
- Receptors, G-Protein-Coupled/genetics
- Receptors, G-Protein-Coupled/metabolism*
- Tissue Distribution
- Wnt Proteins/genetics
- Wnt Proteins/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 20652957 Full text @ Dev. Dyn.
Citation
Jin, J., Morse, M., Frey, C., Petko, J., and Levenson, R. (2010) Expression of GPR177 (Wntless/Evi/Sprinter), a highly conserved Wnt-transport protein, in rat tissues, zebrafish embryos, and cultured human cells. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(9):2426-2434.
Abstract
GPR177 is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. Little is currently known, however, regarding expression of GPR177, especially in vertebrate species. We have developed an antiserum against GPR177, and used it to examine expression of GPR177 in human tissue culture cells, adult mouse, and rat tissues, as well as developing zebrafish embryos. In rodents, GPR177 is expressed in virtually all tissue types and brain regions examined. In zebrafish, GPR177 polypeptides are expressed throughout embryogenesis, and are detectable as early as 1 hr post-fertilization. In situ hybridization analysis reveals that gpr177 mRNA expression is prominent in embryonic zebrafish brain and ear. Structural studies suggest that GPR177 is modified by N-linked sugars, and that the protein contains an even number of transmembrane segments. The relatively ubiquitous expression of GPR177 suggests that this protein may serve to regulate Wnt secretion in a variety of embryonic and adult tissue types.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping