PUBLICATION
JunB-CBF{beta} signaling is essential to maintain sarcomeric Z-disc structure and when defective leads to heart failure
- Authors
- Meder, B., Just, S., Vogel, B., Rudloff, J., Gärtner, L., Dahme, T., Huttner, I., Zankl, A., Katus, H.A., and Rottbauer, W.
- ID
- ZDB-PUB-100719-23
- Date
- 2010
- Source
- Journal of Cell Science 123(Pt 15): 2613-2620 (Journal)
- Registered Authors
- Dahme, Tillmann, Just, Steffen, Meder, Benjamin, Rottbauer, Wolfgang, Rudloff, Jessica, Vogel, Britta
- Keywords
- CBFβ, Z-disc, Heart failure, Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Core Binding Factor beta Subunit/chemistry
- Core Binding Factor beta Subunit/genetics
- Core Binding Factor beta Subunit/metabolism*
- Heart Failure/genetics
- Heart Failure/metabolism
- Humans
- Immunoblotting
- In Situ Hybridization
- Mice
- Microscopy, Electron, Transmission
- Molecular Sequence Data
- Muscle, Skeletal/metabolism
- Muscle, Skeletal/ultrastructure
- Myocardium/metabolism
- Myocardium/ultrastructure
- Myocytes, Cardiac/metabolism
- Polymerase Chain Reaction
- Proto-Oncogene Proteins c-jun/genetics
- Proto-Oncogene Proteins c-jun/metabolism*
- Sarcomeres/genetics
- Sarcomeres/metabolism*
- Sequence Homology, Amino Acid
- Zebrafish
- PubMed
- 20605922 Full text @ J. Cell Sci.
Citation
Meder, B., Just, S., Vogel, B., Rudloff, J., Gärtner, L., Dahme, T., Huttner, I., Zankl, A., Katus, H.A., and Rottbauer, W. (2010) JunB-CBF{beta} signaling is essential to maintain sarcomeric Z-disc structure and when defective leads to heart failure. Journal of Cell Science. 123(Pt 15):2613-2620.
Abstract
In muscle cells, a complex network of Z-disc proteins allows proper reception, transduction and transmission of mechanical and biochemical signals. Mutations in genes encoding different Z-disc proteins such as integrin-linked kinase (ILK) and nexilin have recently been shown to cause heart failure by distinct mechanisms such as disturbed mechanosensing, altered mechanotransduction or mechanical Z-disc destabilization. We identified core-binding factor beta (CBFbeta) as an essential component for maintaining sarcomeric Z-disc and myofilament organization in heart and skeletal muscle. In CBFbeta-deficient cardiomyocytes and skeletal-muscle cells, myofilaments are thinned and Z-discs are misaligned, leading to progressive impairment of heart and skeletal-muscle function. Transcription of the gene encoding CBFbeta mainly depends on JunB activity. In JunB-morphant zebrafish, which show a heart-failure phenotype similar to that of CBFbeta-deficient zebrafish, transcript and protein levels of CBFbeta are severely reduced. Accordingly, ectopic expression of CBFbeta can reconstitute cardiomyocyte function and rescue heart failure in JunB morphants, demonstrating for the first time an essential role of JunB-CBFbeta signaling for maintaining sarcomere architecture and function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping