PUBLICATION
Characterization of behavioral and endocrine effects of LSD on zebrafish
- Authors
- Grossman, L., Utterback, E., Stewart, A., Gaikwad, S., Chung, K.M., Suciu, C., Wong, K., Elegante, M., Elkhayat, S., Tan, J., Gilder, T., Wu, N., Dileo, J., Cachat, J., and Kalueff, A.V.
- ID
- ZDB-PUB-100625-6
- Date
- 2010
- Source
- Behavioural brain research 214(2): 277-284 (Journal)
- Registered Authors
- Cachat, Jonathan, Kalueff, Allan V.
- Keywords
- LSD, Adult zebrafish, Behavioral paradigms, Cortisol, Hallucinogenic drugs, Screening
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects*
- Body Fluids/chemistry
- Dose-Response Relationship, Drug
- Female
- Hallucinogens/pharmacology*
- Hydrocortisone/analysis
- Lysergic Acid Diethylamide/pharmacology*
- Male
- Video Recording/methods
- Zebrafish
- PubMed
- 20561961 Full text @ Behav. Brain Res.
Citation
Grossman, L., Utterback, E., Stewart, A., Gaikwad, S., Chung, K.M., Suciu, C., Wong, K., Elegante, M., Elkhayat, S., Tan, J., Gilder, T., Wu, N., Dileo, J., Cachat, J., and Kalueff, A.V. (2010) Characterization of behavioral and endocrine effects of LSD on zebrafish. Behavioural brain research. 214(2):277-284.
Abstract
Lysergic acid diethylamide (LSD) is a potent hallucinogenic drug that strongly affects animal and human behavior. Although adult zebrafish (Danio rerio) are emerging as a promising neurobehavioral model, the effects of LSD on zebrafish have not been investigated previously. Several behavioral paradigms (the novel tank, observation cylinder, light-dark box, open field, T-maze, social preference and shoaling tests), as well as modern video-tracking tools and whole-body cortisol assay were used to characterize the effects of acute LSD in zebrafish. While lower doses (5-100mug/L) did not affect zebrafish behavior, 250mug/L LSD increased top dwelling and reduced freezing in the novel tank and observation cylinder tests, also affecting spatiotemporal patterns of activity (as assessed by 3D reconstruction of zebrafish traces and ethograms). LSD evoked mild thigmotaxis in the open field test, increased light behavior in the light-dark test, reduced the number of arm entries and freezing in the T-maze and social preference test, without affecting social preference. In contrast, LSD affected zebrafish shoaling (increasing the inter-fish distance in a group), and elevated whole-body cortisol levels. Overall, our findings show sensitivity of zebrafish to LSD action, and support the use of zebrafish models to study hallucinogenic drugs of abuse.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping