PUBLICATION

Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions

Authors
Yamamoto, M., Morita, R., Mizoguchi, T., Matsuo, H., Isoda, M., Ishitani, T., Chitnis, A.B., Matsumoto, K., Crump, J.G., Hozumi, K., Yonemura, S., Kawakami, K., and Itoh, M.
ID
ZDB-PUB-100625-27
Date
2010
Source
Development (Cambridge, England)   137(15): 2527-2537 (Journal)
Registered Authors
Chitnis, Ajay, Crump, Gage DeKoeyer, Ishitani, Tohru, Itoh, Motoyuki, Kawakami, Koichi, Mizoguchi, Takamasa
Keywords
Mind bomb, Notch signalling, Notochord, Basement membrane, Muscle patterning, Zebrafish
MeSH Terms
  • 3T3 Cells
  • Animals
  • Body Patterning*
  • COS Cells
  • Calcium-Binding Proteins/metabolism*
  • Cell Lineage*
  • Chlorocebus aethiops
  • Endocytosis
  • Gene Expression Regulation, Developmental*
  • Intercellular Signaling Peptides and Proteins/metabolism*
  • Membrane Proteins/metabolism*
  • Mice
  • Models, Biological
  • Notochord/physiology*
  • Receptors, Notch/metabolism*
  • Two-Hybrid System Techniques
  • Ubiquitin/metabolism
  • Ubiquitin-Protein Ligases/metabolism*
  • Zebrafish
  • Zebrafish Proteins/metabolism*
PubMed
20573700 Full text @ Development
Abstract
In the developing embryo, cell-cell signalling is necessary for tissue patterning and structural organization. During midline development, the notochord plays roles in the patterning of its surrounding tissues while forming the axial structure; however, how these patterning and structural roles are coordinated remains elusive. Here, we identify a mechanism by which Notch signalling regulates the patterning activities and structural integrity of the notochord. We found that Mind bomb (Mib) ubiquitylates Jagged 1 (Jag1) and is essential in the signal-emitting cells for Jag1 to activate Notch signalling. In zebrafish, loss- and gain-of-function analyses showed that Mib-Jag1-Notch signalling favours the development of non-vacuolated cells at the expense of vacuolated cells in the notochord. This leads to changes in the peri-notochordal basement membrane formation and patterning surrounding the muscle pioneer cells. These data reveal a previously unrecognized mechanism regulating the patterning and structural roles of the notochord by Mib-Jag1-Notch signalling-mediated cell-fate determination.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes