ZFIN ID: ZDB-PUB-100625-27
Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions
Yamamoto, M., Morita, R., Mizoguchi, T., Matsuo, H., Isoda, M., Ishitani, T., Chitnis, A.B., Matsumoto, K., Crump, J.G., Hozumi, K., Yonemura, S., Kawakami, K., and Itoh, M.
Date: 2010
Source: Development (Cambridge, England)   137(15): 2527-2537 (Journal)
Registered Authors: Chitnis, Ajay, Crump, Gage DeKoeyer, Ishitani, Tohru, Itoh, Motoyuki, Kawakami, Koichi, Mizoguchi, Takamasa
Keywords: Mind bomb, Notch signalling, Notochord, Basement membrane, Muscle patterning, Zebrafish
MeSH Terms:
  • 3T3 Cells
  • Animals
  • Body Patterning*
  • COS Cells
  • Calcium-Binding Proteins/metabolism*
  • Cell Lineage*
  • Chlorocebus aethiops
  • Endocytosis
  • Gene Expression Regulation, Developmental*
  • Intercellular Signaling Peptides and Proteins/metabolism*
  • Membrane Proteins/metabolism*
  • Mice
  • Models, Biological
  • Notochord/physiology*
  • Receptors, Notch/metabolism*
  • Two-Hybrid System Techniques
  • Ubiquitin/metabolism
  • Ubiquitin-Protein Ligases/metabolism*
  • Zebrafish
  • Zebrafish Proteins/metabolism*
PubMed: 20573700 Full text @ Development
In the developing embryo, cell-cell signalling is necessary for tissue patterning and structural organization. During midline development, the notochord plays roles in the patterning of its surrounding tissues while forming the axial structure; however, how these patterning and structural roles are coordinated remains elusive. Here, we identify a mechanism by which Notch signalling regulates the patterning activities and structural integrity of the notochord. We found that Mind bomb (Mib) ubiquitylates Jagged 1 (Jag1) and is essential in the signal-emitting cells for Jag1 to activate Notch signalling. In zebrafish, loss- and gain-of-function analyses showed that Mib-Jag1-Notch signalling favours the development of non-vacuolated cells at the expense of vacuolated cells in the notochord. This leads to changes in the peri-notochordal basement membrane formation and patterning surrounding the muscle pioneer cells. These data reveal a previously unrecognized mechanism regulating the patterning and structural roles of the notochord by Mib-Jag1-Notch signalling-mediated cell-fate determination.