ZFIN ID: ZDB-PUB-100625-20
Ultraviolet radiation and handling medium osmolarity affect chimaerism success in zebrafish
Francisco-Simão, M., Cardona-Costa, J., Pérez-Camps, M., and García-Ximénez, F.
Date: 2011
Source: Zygote (Cambridge, England)   19(1): 67-70 (Journal)
Registered Authors: Cardona-Costa, Jose
Keywords: Embryo, Germ-line chimaerism, Osmolarity, Zebrafish
MeSH Terms:
  • Animals
  • Chimerism/drug effects*
  • Chimerism/embryology*
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/radiation effects
  • Osmolar Concentration
  • Ultraviolet Rays*
  • Zebrafish/embryology*
PubMed: 20569510 Full text @ Zygote
ABSTRACT
The effects of a predefined ultraviolet radiation dose (0.529 mW/cm2 for 30s) together with two different micromanipulation medium osmolarities (30 mOsm/kg vs 300 mOsm/kg) were tested on embryo survival at different developmental stages and on the somatic (skin) and germ-line chimaerism rates. Somatic (13%, 6/47 adults) and germ-line chimaerism (50% pigmented F1 larvae) were detected only in the UV-treated recipient embryos micromanipulated in a 300 mOsm/kg medium. From the results obtained, we concluded that the conditions cited above were the most suitable to improve somatic and germ-line chimaerism rates in zebrafish.
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