PUBLICATION
Long-term warm or cold acclimation elicits a specific transcriptional response and affects energy metabolism in zebrafish
- Authors
- Vergauwen, L., Benoot, D., Blust, R., and Knapen, D.
- ID
- ZDB-PUB-100621-3
- Date
- 2010
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 157(2): 149-157 (Journal)
- Registered Authors
- Blust, Ronny, Knapen, Dries, Vergauwen, Lucia
- Keywords
- Condition factor, Energy stores, Microarrays, Temperature, Transcriptomics, Zebrafish
- Datasets
- GEO:GSE17756
- MeSH Terms
-
- Acclimatization/physiology*
- Animals
- Cold Temperature*
- Energy Metabolism/genetics*
- Gene Expression Profiling
- Gene Expression Regulation
- HSP70 Heat-Shock Proteins/genetics
- HSP70 Heat-Shock Proteins/metabolism
- Hot Temperature*
- Liver/metabolism
- Oligonucleotide Array Sequence Analysis
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Stress, Physiological/genetics
- Time Factors
- Transcription, Genetic*
- Zebrafish/genetics*
- Zebrafish/metabolism*
- PubMed
- 20541617 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Vergauwen, L., Benoot, D., Blust, R., and Knapen, D. (2010) Long-term warm or cold acclimation elicits a specific transcriptional response and affects energy metabolism in zebrafish. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 157(2):149-157.
Abstract
Organisms are often forced to acclimate to changing environmental temperature. Temperature compensation mechanisms have been reported, which enable organisms to minimize some of the temperature related effects. To investigate this process, zebrafish (Danio rerio) were acclimated to a control (26 degrees C), an increased (34 degrees C) or a decreased (18 degrees C) temperature for 4, 14 and 28days. In general, warm acclimation depleted energy stores and decreased the condition factor, while cold acclimation increased both. The energy parameters as well as the transcriptional responses (investigated using printed 15k microarrays and real time PCR) indicated that warm acclimation was particularly stressful. However, after 28days of warm acclimation, energy stores had recovered from the initial depletion. This could have been facilitated by the observed downregulation of transcripts involved in catabolic processes. Transcriptional regulation seemed to be an important means of coordinating the temperature compensation process. We could distinguish an early response which was independent of the direction of the temperature change and a direction specific long-term response. The early response was characterized by the upregulation of defence mechanisms, tissue regeneration and hemopoiesis. In the long-term response there was a strong emphasis on compensating for the altered metabolic rate as well as cell structure and replacement.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping