PUBLICATION

Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos

Authors
Alexandre, D., Alonzeau, J., Bill, B.R., Ekker, S.C., and Waschek, J.A.
ID
ZDB-PUB-100614-17
Date
2011
Source
Journal of molecular neuroscience : MN   43(1): 94-100 (Journal)
Registered Authors
Bill, Brent, Ekker, Stephen C.
Keywords
Zebrafish, PACAP receptors, Ontogeny, Central nervous system
MeSH Terms
  • Animals
  • Brain/embryology
  • Brain/metabolism
  • Embryo, Nonmammalian/anatomy & histology
  • Embryo, Nonmammalian/metabolism*
  • Gene Expression
  • In Situ Hybridization
  • Pituitary Adenylate Cyclase-Activating Polypeptide/genetics*
  • Pituitary Adenylate Cyclase-Activating Polypeptide/metabolism
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I/genetics*
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed
20526695 Full text @ J. Mol. Neurosci.
Abstract
This study describes the expression of the pituitary adenylate cyclase-activating polypeptide (PACAP1 and PACAP2) and PAC1 receptor genes (PAC1a-R and PAC1b-R) in the brain of zebrafish (Danio rerio) during development. In situ hybridization of the 24- and 48-hpf embryos revealed that PACAP genes were expressed in the telencephalon, the diencephalon, the rhombencephalon, and the neurons in the dorsal part of the spinal cord. PACAP2 mRNA appears to be the most abundant form during brain development. The two PAC1-R subtypes showed a similar expression pattern: mRNAs were detected in the forebrain, the thalamus, and the rhombencephalon. However, in the tectum, only PAC1b-R gene was detected. These results suggest that, in fish, PACAP may play a role in brain development.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping