PUBLICATION

Zebrafish sp7:EGFP: A transgenic for studying otic vesicle formation, skeletogenesis, and bone regeneration

Authors
Delaurier, A., Eames, B.F., Blanco-Sánchez, B., Peng, G., He, X., Swartz, M.E., Ullmann, B., Westerfield, M., and Kimmel, C.B.
ID
ZDB-PUB-100601-19
Date
2010
Source
Genesis (New York, N.Y. : 2000)   48(8): 505-511 (Journal)
Registered Authors
Blanco, Bernardo, DeLaurier, April, Eames, Brian F., He, Xinjun, Kimmel, Charles B., Peng, Gang, Swartz, Mary, Ullmann, Bonnie, Westerfield, Monte
Keywords
bone, Danio rerio, gene transfer techniques, otic vesicle, regeneration, sp7
MeSH Terms
  • Animal Fins/physiology
  • Animal Fins/surgery
  • Animals
  • Animals, Genetically Modified
  • Bone Regeneration/genetics*
  • Chondrocytes/metabolism
  • Chromosomes, Artificial, Bacterial/genetics
  • Cloning, Molecular
  • Ear/embryology
  • Ear/growth & development
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • In Situ Hybridization
  • Larva/genetics
  • Larva/metabolism
  • Organogenesis/genetics*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
20506187 Full text @ Genesis
Abstract
We report the expression pattern and construction of a transgenic zebrafish line for a transcription factor involved in otic vesicle formation and skeletogenesis. The zinc finger transcription factor sp7 (formerly called osterix) is reported as a marker of osteoblasts. Using bacterial artificial chromosome (BAC)-mediated transgenesis, we generated a zebrafish transgenic line for studying skeletal development, Tg(sp7:EGFP)b1212. Using a zebrafish BAC, EGFP was introduced downstream of the regulatory regions of sp7 and injected into 1 cell-stage embryos. In this transgenic line, GFP expression reproduces endogenous sp7 gene expression in the otic placode and vesicle, and in forming skeletal structures. GFP-positive cells were also detected in adult fish, and were found associated with regenerating fin rays post-amputation. This line provides an essential tool for the further study of zebrafish otic vesicle formation and the development and regeneration of the skeleton.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes