ZFIN ID: ZDB-PUB-100601-19
Zebrafish sp7:EGFP: A transgenic for studying otic vesicle formation, skeletogenesis, and bone regeneration
Delaurier, A., Eames, B.F., Blanco-Sánchez, B., Peng, G., He, X., Swartz, M.E., Ullmann, B., Westerfield, M., and Kimmel, C.B.
Date: 2010
Source: Genesis (New York, N.Y. : 2000)   48(8): 505-511 (Journal)
Registered Authors: Blanco, Bernardo, DeLaurier, April, Eames, Brian F., He, Xinjun, Kimmel, Charles B., Peng, Gang, Swartz, Mary, Ullmann, Bonnie, Westerfield, Monte
Keywords: bone, Danio rerio, gene transfer techniques, otic vesicle, regeneration, sp7
MeSH Terms:
  • Animal Fins/physiology
  • Animal Fins/surgery
  • Animals
  • Animals, Genetically Modified
  • Bone Regeneration/genetics*
  • Chondrocytes/metabolism
  • Chromosomes, Artificial, Bacterial/genetics
  • Cloning, Molecular
  • Ear/embryology
  • Ear/growth & development
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • In Situ Hybridization
  • Larva/genetics
  • Larva/metabolism
  • Organogenesis/genetics*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 20506187 Full text @ Genesis
We report the expression pattern and construction of a transgenic zebrafish line for a transcription factor involved in otic vesicle formation and skeletogenesis. The zinc finger transcription factor sp7 (formerly called osterix) is reported as a marker of osteoblasts. Using bacterial artificial chromosome (BAC)-mediated transgenesis, we generated a zebrafish transgenic line for studying skeletal development, Tg(sp7:EGFP)b1212. Using a zebrafish BAC, EGFP was introduced downstream of the regulatory regions of sp7 and injected into 1 cell-stage embryos. In this transgenic line, GFP expression reproduces endogenous sp7 gene expression in the otic placode and vesicle, and in forming skeletal structures. GFP-positive cells were also detected in adult fish, and were found associated with regenerating fin rays post-amputation. This line provides an essential tool for the further study of zebrafish otic vesicle formation and the development and regeneration of the skeleton.