PUBLICATION

Ovo1 links Wnt signaling with N-cadherin localization during neural crest migration

Authors
Piloto, S., and Schilling, T.F.
ID
ZDB-PUB-100518-8
Date
2010
Source
Development (Cambridge, England)   137(12): 1981-1990 (Journal)
Registered Authors
Schilling, Tom
Keywords
Neural crest, N-cadherin, Wnt, Zebrafish
Datasets
GEO:GSE21539
MeSH Terms
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism
  • Neural Crest/cytology*
  • Neural Crest/metabolism
  • Neural Crest/physiology
  • Cell Adhesion
  • Cell Movement/physiology*
  • Neurons/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Signal Transduction/physiology*
  • Animals
  • Cytoplasm/metabolism
  • Cadherins*/biosynthesis
  • Cadherins*/metabolism
  • Cadherins*/physiology
(all 16)
PubMed
20463035 Full text @ Development
Abstract
A fundamental issue in cell biology is how migratory cell behaviors are controlled by dynamically regulated cell adhesion. Vertebrate neural crest (NC) cells rapidly alter cadherin expression and localization at the cell surface during migration. Secreted Wnts induce some of these changes in NC adhesion and also promote specification of NC-derived pigment cells. Here, we show that the zebrafish transcription factor Ovo1 is a Wnt target gene that controls migration of pigment precursors by regulating the intracellular movements of N-cadherin (Ncad). Ovo1 genetically interacts with Ncad and its depletion causes Ncad to accumulate inside cells. Ovo1-deficient embryos strongly upregulate factors involved in intracellular trafficking, including several rab GTPases, known to modulate cellular localization of cadherins. Surprisingly, NC cells express high levels of many of these rab genes in the early embryo, chemical inhibitors of Rab functions rescue NC development in Ovo1-deficient embryos and overexpression of a Rab-interacting protein leads to similar defects in NC migration. These results suggest that Ovo proteins link Wnt signaling to intracellular trafficking pathways that localize Ncad in NC cells and allow them to migrate. Similar processes probably occur in other cell types in which Wnt signaling promotes migration.
Genes / Markers
Figures
Figure Gallery (16 images) / 2
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-cdh2 + MO2-ovol1astandard conditionsPrim-5
WT + MO2-ovol1astandard conditions50%-epiboly to Bud
WT + MO2-ovol1astandard conditionsPrim-5
WT + MO2-ovol1astandard conditionsPrim-5
WT + MO2-ovol1astandard conditionsPrim-5
cdh2r210/+ + MO2-ovol1astandard conditionsPrim-5
1 - 6 of 6
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ir937TgTransgenic Insertion
    r210
      		Point Mutation
      w26TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        cdh2MO1-cdh2MRPHLNO
        ovol1aMO1-ovol1aMRPHLNO
        ovol1aMO2-ovol1aMRPHLNO
        rab11fip2MO1-rab11fip2MRPHLNO
        tp53MO1-tp53MRPHLNO
        1 - 5 of 5
        Show
        Fish
        Fish
        cdh2r210/+
        cdh2r210/+ + MO2-ovol1a
        ir937Tg
        w26Tg/+
        WT
        WT + MO1-cdh2 + MO2-ovol1a
        WT + MO2-ovol1a
        1 - 7 of 7
        Show
        Antibodies
        No data available
        Orthology
        Gene Orthology
        ovol1a
        1 - 1 of 1
        Show
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        GFPEFGGFP
        1 - 2 of 2
        Show
        Mapping
        No data available
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        Citation
        Piloto, S., and Schilling, T.F. (2010) Ovo1 links Wnt signaling with N-cadherin localization during neural crest migration. Development (Cambridge, England). 137(12):1981-1990.