PUBLICATION

Photoactivation of the CreER(T2) Recombinase for Conditional Site-Specific Recombination with High Spatiotemporal Resolution

Authors
Sinha, D.K., Neveu, P., Gagey, N., Aujard, I., Le Saux, T., Rampon, C., Gauron, C., Kawakami, K., Leucht, C., Bally-Cuif, L., Volovitch, M., Bensimon, D., Jullien, L., and Vriz, S.
ID
ZDB-PUB-100511-8
Date
2010
Source
Zebrafish   7(2): 199-204 (Journal)
Registered Authors
Bally-Cuif, Laure, Kawakami, Koichi, Leucht, Christoph, Vriz, Sophie
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • DNA Primers/genetics
  • Gene Expression Regulation, Enzymologic/physiology*
  • HSP70 Heat-Shock Proteins/metabolism
  • Integrases/metabolism*
  • Microscopy, Fluorescence
  • Photochemical Processes
  • Polymerase Chain Reaction
  • Recombinant Fusion Proteins/metabolism*
  • Recombination, Genetic/physiology*
  • Spectrometry, Fluorescence
  • Zebrafish*
PubMed
20441524 Full text @ Zebrafish
Abstract
We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreER(T2) fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales).
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes