PUBLICATION

Transcription Intermediary Factor 1gamma Decreases Protein Expression of the Transcriptional Cofactor, LIM-Domain-Binding 1

Authors
Howard, P.W., Ransom, D.G., and Maurer, R.A.
ID
ZDB-PUB-100511-16
Date
2010
Source
Biochemical and Biophysical Research Communications   396(3): 674-678 (Journal)
Registered Authors
Ransom, David G.
Keywords
Ubiquitin ligase, LIM-homeodomain transcription factor, Proteasome
MeSH Terms
  • Animals
  • CHO Cells
  • Cell Line
  • Cricetinae
  • Cricetulus
  • DNA-Binding Proteins/biosynthesis*
  • DNA-Binding Proteins/genetics
  • Gene Expression Regulation
  • Gene Knockdown Techniques
  • Humans
  • Immunoprecipitation
  • LIM Domain Proteins
  • Mice
  • Promoter Regions, Genetic
  • Transcription Factors/biosynthesis*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed
20447379 Full text @ Biochem. Biophys. Res. Commun.
Abstract
LIM-domain-binding 1 (LDB1) is a cofactor that participates in formation of regulatory complexes involving transcription factors containing LIM domains as well as other factors. We have examined the ability of transcriptional Intermediary factor 1gamma (TIF1gamma) to decrease LDB1 expression. An expression vector for TIF1gamma was found to decrease expression of LDB1. A mutation which disrupts the ubiquitin ligase activity of TIF1gamma was found to block the ability of TIF1gamma to decrease LDB1 expression. Proteasome inhibitors were also able to block TIF1gamma effects on LDB1. Immunoprecipitation studies provided evidence that LDB1 interacts with TIF1gamma in intact cells. Knockdown of TIF1gamma in zebrafish embryos led to increased expression of LDB1 providing evidence for a physiological role of TIF1gamma in regulating LDB1 expression. Reporter gene assays demonstrated that TIF1gamma can alter the activity of LIM-homeodomain transcription factor-responsive promoters. These studies are consistent with a model in which TIF1gamma acts to ubiquitinate LDB1 leading to degradation of LDB1 and changes in transcription of LDB1-dependent promoters.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes