PUBLICATION
Cardiovascular and respiratory developmental plasticity under oxygen depleted environment and in genetically hypoxic zebrafish (Danio rerio)
- Authors
- Yaqoob, N., and Schwerte, T.
- ID
- ZDB-PUB-100408-23
- Date
- 2010
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 156(4): 475-484 (Journal)
- Registered Authors
- Schwerte, Thorsten
- Keywords
- Hypoxia, Anoxia, Zebrafish, Viability, Cardio-vascular system, Hyperventilation
- MeSH Terms
-
- Adaptation, Physiological/physiology*
- Animals
- Blood Vessels/anatomy & histology
- Blood Vessels/physiology
- Cardiovascular System/growth & development*
- Hypoxia/genetics*
- Hypoxia/metabolism
- Mutation
- Oxygen/metabolism*
- Oxygen Consumption/genetics
- Oxygen Consumption/physiology
- Respiratory System/growth & development*
- Zebrafish/anatomy & histology
- Zebrafish/blood
- Zebrafish/genetics*
- Zebrafish/physiology*
- PubMed
- 20363352 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Yaqoob, N., and Schwerte, T. (2010) Cardiovascular and respiratory developmental plasticity under oxygen depleted environment and in genetically hypoxic zebrafish (Danio rerio). Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 156(4):475-484.
Abstract
Known vertebrate response to low oxygen concentration include change in carbohydrate metabolism, increase in nitric oxide, stimulation of red blood cell and hemoglobin production and induction of gene expression for glycolytic enzymes and hormones. Also, extreme hypoxia plays main role in pathological studies of cardiac dysfunction. The morphological and physiological developmental studies of the cardiovascular system under low oxygen are important as it is directly related to oxygen supply and consumption. Furthermore, cardiac function demands high energy during system development and thus it is most likely to be affected by hypoxia. Zebrafish (Danio rerio) can act as a model organism for oxygen demand management study as in natural environment, due to ecological disturbances, it is exposed to changes in oxygen concentrations routinely and thus would have natural ability to cope with it for survival. We have studied, in zebrafish, i) cardiovascular flexibility under extreme hypoxia (PO(2)=20torr, 3kPa) at 3-10dpf (days post-fertilization), ii) cardiac re-animation in normoxia (PO(2)=152torr, 20kPa) after 90min of anoxia (PO(2)=0torr, 0kPa)-induced suspended animation at 4dpf and iii) oxygen consumption in 8dpf von Hippel-Lindau (vhl(-/-)) mutant that exhibits an artificial hypoxic response under normoxic conditions. In hypoxic fish, cardiac output, stroke volume and end-diastolic volume were elevated while intersegmental blood vessels vascularization index at 6dpf and at 10dpf was 22% and 11% higher respectively as compared to the normoxic fish. The heart rate in hypoxic fish was lower until 6dpf and then showed an elevated trend. There was no significant difference in body length between the hypoxic and normoxic individuals. The observed changes may have enhanced the performance of the cardiovascular system for oxygen uptake. We also report for the first time that the post-anoxia re-animated heart rate returns to normal after 48hours. Measurement of oxygen consumption in 8dpf hyper-ventilating vhl(-/-) mutant was, unexpectedly, significantly lower than the non-mutant fish of the same age which point towards artificial hypoxic signal from brain in these mutants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping