ZFIN ID: ZDB-PUB-100330-35
Characterization of neural stem cells and their progeny in the adult zebrafish optic tectum
Ito, Y., Tanaka, H., Okamoto, H., and Ohshima, T.
Date: 2010
Source: Developmental Biology   342(1): 26-38 (Journal)
Registered Authors: Okamoto, Hitoshi, Tanaka, Hideomi
Keywords: Adult neurogenesis, Neural stem cells, Optic tectum, Radial glia, Zebrafish
MeSH Terms:
  • Adult
  • Animals
  • Animals, Genetically Modified
  • Brain/cytology
  • Brain/metabolism*
  • Bromodeoxyuridine/metabolism
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology
  • Glial Fibrillary Acidic Protein/genetics
  • Glial Fibrillary Acidic Protein/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Humans
  • Multipotent Stem Cells/metabolism
  • Neuroglia/cytology
  • Neuroglia/metabolism*
  • Neurons/cytology
  • Neurons/metabolism*
  • Stem Cells/cytology
  • Stem Cells/metabolism*
  • Stem Cells/physiology
  • Superior Colliculi/metabolism*
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed: 20346355 Full text @ Dev. Biol.
In the adult teleost brain, proliferating cells are observed in a broad area, while these cells have a restricted distribution in adult mammalian brains. In the adult teleost optic tectum, most of the proliferating cells are distributed in the caudal margin of the periventricular gray zone (PGZ). We found that the PGZ is largely divided into 3 regions: 1 mitotic region and 2 post-mitotic regions-the superficial and deep layers. These regions are distinguished by the differential expression of several marker genes: pcna, sox2, msi1, elavl3, gfap, fabp7a, and s100beta. Using transgenic zebrafish Tg (gfap:GFP), we found that the deep layer cells specifically express gfap:GFP and have a radial glial morphology. We noted that bromodeoxyuridine (BrdU)-positive cells in the mitotic region did not exhibit glial properties, but maintained neuroepithelial characteristics. Pulse-chase experiments with BrdU-positive cells revealed the presence of self-renewing stem cells within the mitotic region. BrdU-positive cells differentiate into glutamatergic or GABAergic neurons and oligodendrocytes in the superficial layer and into radial glial cells in the deep layer. These results demonstrate that the proliferating cells in the PGZ contribute to neuronal and glial lineages to maintain the structure of the optic tectum in adult zebrafish.