PUBLICATION
Exploring microRNA functions in zebrafish
- Authors
- Fjose, A., and Zhao, X.F.
- ID
- ZDB-PUB-100317-16
- Date
- 2010
- Source
- New biotechnology 27(3): 250-255 (Review)
- Registered Authors
- Fjose, Anders
- Keywords
- none
- MeSH Terms
-
- Animals
- Gene Silencing*
- Humans
- MicroRNAs/genetics*
- MicroRNAs/metabolism*
- RNA Interference
- RNA, Small Interfering/genetics
- RNA, Small Interfering/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 20219709 Full text @ N. Biotechnol.
Citation
Fjose, A., and Zhao, X.F. (2010) Exploring microRNA functions in zebrafish. New biotechnology. 27(3):250-255.
Abstract
The microRNA (miRNA) pathway and the phenomenon of RNA interference (RNAi) have similar mechanisms and depend partly on the same cellular factors. Humans may have more than 1000 different miRNAs, which are essential regulators of gene expression in many biological processes. RNAi-based techniques have become important tools in biomedical research and have great potential for use in disease therapy. Zebrafish (Danio rerio) provide several advantages for the study of miRNA functions. However, reliable RNAi-based gene silencing techniques have not been established for this important vertebrate model. In this article, we describe the highly efficient in vivo assays used to study miRNA functions in zebrafish and how these experimental approaches have uncovered new regulatory mechanisms in vertebrates. This research has also provided information that explains why RNAi techniques have been unsuccessful in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping