PUBLICATION

Pseudoloma neurophilia infections in zebrafish Danio rerio: effects of stress on survival, growth, and reproduction

Authors
Ramsay, J.M., Watral, V., Schreck, C.B., and Kent, M.L.
ID
ZDB-PUB-100302-13
Date
2009
Source
Diseases of aquatic organisms   88(1): 69-84 (Journal)
Registered Authors
Kent, Michael
Keywords
Pseudoloma neurophilia, Stress, Growth, Reproduction, Mortality, Microsporidia
MeSH Terms
  • Animals
  • Fish Diseases/microbiology*
  • Fish Diseases/mortality
  • Fish Diseases/physiopathology
  • Hydrocortisone
  • Microsporidia*
  • Microsporidiosis/mortality
  • Microsporidiosis/physiopathology
  • Microsporidiosis/veterinary*
  • Reproduction
  • Stress, Physiological*
  • Zebrafish*
PubMed
20183967 Full text @ Dis. Aquat. Organ.
Abstract
Pseudoloma neurophilia (Microsporidia) is a common disease of zebrafish Danio rerio, including those used as research models. We conducted a study comprised of 4 separate experiments to determine the effects of husbandry stress on preexisting and experimental P. neurophilia infections and the subsequent effects on survival, infection onset and intensity, fish growth, and reproduction. In fish (AB strain) with preexisting infections, stress or feeding cortisol significantly increased mortality over 7 wk compared to no stress or cortisol treatment. In contrast, no mortality was observed in fish (TL strain) experimentally exposed to P. neurophilia over 10 wk. A third experiment involved experimental exposure of AB fish to P. neurophilia and exposure to crowding and handling stressors. No mortality was associated with P. neurophilia regardless of stress treatment over a period of 20 wk. However, the onset of infection occurred sooner in stress-treated fish. Stress significantly increased the mean intensity of infection (described as xenoma area/spinal cord area in histological sections) at Week 20 post-exposure (PE). In fish with preexisting infections, myositis was significantly greater in stressed and cortisol-treated fish than those not stressed. With experimental exposure of AB fish, stressed and infected groups weighed significantly less than the control group at Week 20 PE. Regarding fecundity, the number of larvae hatched at 5 d post fertilization was negatively associated with mean infection intensity among P. neurophilia-infected and stressed AB fish. These experiments are the first to show empirically that P. neurophilia can be associated with reduced weight and fecundity, and that stress can exacerbate the severity of the infection.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes