ZFIN ID: ZDB-PUB-100223-14
Neuronal Regulation of the Spatial Patterning of Neurogenesis
Gonzalez-Quevedo, R., Lee, Y., Poss, K.D., and Wilkinson, D.G.
Date: 2010
Source: Developmental Cell   18(1): 136-147 (Journal)
Registered Authors: Lee, Yoonsung, Poss, Kenneth D., Wilkinson, David
MeSH Terms:
  • Animals
  • Body Patterning/physiology*
  • Cell Communication/physiology
  • Cytochrome P-450 Enzyme System/genetics
  • Cytochrome P-450 Enzyme System/metabolism
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Fibroblast Growth Factor 2/metabolism
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism
  • Gene Expression Regulation, Developmental/physiology
  • Neurogenesis/physiology*
  • Neurons/cytology
  • Neurons/metabolism*
  • Rhombencephalon/cytology
  • Rhombencephalon/embryology*
  • Rhombencephalon/metabolism*
  • SOX9 Transcription Factor/genetics
  • SOX9 Transcription Factor/metabolism
  • Signal Transduction/physiology
  • Stem Cells/cytology
  • Stem Cells/metabolism*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 20152184 Full text @ Dev. Cell
Precise regulation of neurogenesis is achieved in specific regions of the vertebrate nervous system by formation of distinct neurogenic and nonneurogenic zones. We have investigated how neurogenesis becomes confined to zones adjacent to rhombomere boundaries in the zebrafish hindbrain. The nonneurogenic zone at segment centers comprises a distinct progenitor population that expresses fibroblast growth factor (fgfr) 2, erm, sox9b, and the retinoic acid degrading enzyme, cyp26b1. FGF receptor activation upregulates expression of these genes and inhibits neurogenesis in segment centers. Cyp26 activity is a key effector inhibiting neuronal differentiation, suggesting antagonistic interactions with retinoid signaling. We identify the critical FGF ligand, fgf20a, which is expressed by specific neurons located in the mantle region at the center of segments, adjacent to the nonneurogenic zone. Fgf20a mutants have ectopic neurogenesis and lack the segment center progenitor population. Our findings reveal how signaling from neurons induces formation of a nonneurogenic zone of neural progenitors.