ZFIN ID: ZDB-PUB-100202-5
Localization of c-mos mRNA around the animal pole in the zebrafish oocyte with Zor-1/Zorba
Suzuki, H., Tsukahara, T., and Inoue, K.
Date: 2009
Source: Bioscience trends   3(3): 96-104 (Journal)
Registered Authors: Inoue, Kunio
Keywords: CPEB, c-mos, CELF/Bruno, RNA localization, oocyte maturation
MeSH Terms:
  • Animals
  • Blotting, Northern
  • Cell Polarity/physiology
  • Cloning, Molecular
  • DNA Primers/genetics
  • DNA, Complementary/genetics
  • Genes, mos/genetics*
  • Immunoprecipitation
  • In Situ Hybridization
  • Oocytes/cytology
  • Oocytes/metabolism*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism*
  • RNA-Binding Proteins/metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/embryology*
  • Zebrafish Proteins/metabolism*
PubMed: 20103830
FIGURES
ABSTRACT
In oocytes, many maternally supplied products are stored, and these products play important roles in cell cycle regulation and early development. Mos protein, which is coded on the c-mos gene, promotes oocyte maturation and is involved in MAP-kinase signaling pathway. In Xenopus, maternally supplied c-mos mRNA undergoes poly(A) addition, and translational activation via CPE (cytoplasmic polyadenylation element) and CPEB (CPE binding protein). The elongated poly(A) is shortened and the c-mos mRNA is degraded during early embryogenesis via EDEN (embryo deadenylation element) and EDEN-BP (EDEN-binding protein). We cloned the full-length zebrafish c-mos gene, which is conserved at the protein coding region in vertebrates. c-mos mRNA has two putative CPE sequences in its 3'UTR, which binds to zebrafish CPEB homologous protein, Zor-1. We could not observe EDEN sequence, and could not detect interaction between c-mos mRNA and zebrafish EDEN-BP homologous protein, Brul, even though immuno precipitation and RT-PCR experiments suggested that c-mos mRNA interacts with Zor-1 in vivo. Interestingly, we found c-mos mRNA is located in the animal cortex of zebrafish oocyte, where Zor-1 protein exists. Taken together, these results suggest that the animal cortex is the central core of oocyte maturation in zebrafish.
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