PUBLICATION
Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity
- Authors
- Verma, A., Bhattacharya, R., Remadevi, I., Li, K., Pramanik, K., Samant, G.V., Horswill, M., Chun, C.Z., Zhao, B., Wang, E., Miao, R.Q., Mukhopadhyay, D., Ramchandran, R., and Wilkinson, G.A.
- ID
- ZDB-PUB-100126-5
- Date
- 2010
- Source
- Blood 115(22): 4614-4622 (Journal)
- Registered Authors
- Ramchandran, Ramani, Verma, Anjali
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis
- Base Sequence
- Blood Vessels/embryology*
- Blood Vessels/metabolism*
- Cell Movement
- Cell Proliferation
- Cells, Cultured
- Chemotaxis/physiology*
- DNA Primers/genetics
- Endothelial Cells/metabolism
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- Humans
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Signal Transduction
- Transfection
- Vascular Endothelial Growth Factor Receptor-2/antagonists & inhibitors
- Vascular Endothelial Growth Factor Receptor-2/genetics
- Vascular Endothelial Growth Factor Receptor-2/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 20086248 Full text @ Blood
Citation
Verma, A., Bhattacharya, R., Remadevi, I., Li, K., Pramanik, K., Samant, G.V., Horswill, M., Chun, C.Z., Zhao, B., Wang, E., Miao, R.Q., Mukhopadhyay, D., Ramchandran, R., and Wilkinson, G.A. (2010) Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity. Blood. 115(22):4614-4622.
Abstract
Endothelial Cell-Specific Chemotaxis Receptor (ECSCR) is a cell surface protein expressed by blood endothelial cells with roles in endothelial cell migration and signal transduction. We investigated the function of ecscr in the development of the zebrafish vasculature. Zebrafish ecscr is expressed in angioblasts and in axial vessels during angioblast migration and vasculogenesis. Morpholino directed ecscr knockdown resulted in defective angioblast migration in the posterior lateral plate mesoderm, a process known to depend on VEGF. In cultured cells, transfected ECSCR localized to actin rich membrane protrusions, co-localizing with KDR/VEGF receptor 2 in these regions. ECSCR silenced cells show reduced VEGF-induced phosphorylation of KDR but not of FLT1/VEGF receptor 1. Finally, chemical inhibition of VEGF receptor activity in zebrafish resulted in angioblast deficiencies which partially overlap with those seen in ecscr morphants. We propose that ecscr promotes migration of zebrafish angioblasts by enhancing endothelial kdr sensitivity to VEGF.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping