PUBLICATION
Effect of Vascular Cadherin Knockdown on Zebrafish Vasculature during Development
- Authors
- Mitchell, I.C., Brown, T.S., Terada, L.S., Amatruda, J.F., and Nwariaku, F.E.
- ID
- ZDB-PUB-100126-18
- Date
- 2010
- Source
- PLoS One 5(1): e8807 (Journal)
- Registered Authors
- Amatruda, James F.
- Keywords
- none
- MeSH Terms
-
- Zebrafish/embryology*
- Antigens, CD/genetics
- Antigens, CD/metabolism*
- Base Sequence
- In Situ Hybridization
- DNA Primers
- Animals
- Gene Knockdown Techniques
- Blood Vessels/embryology*
- Blood Vessels/metabolism
- Animals, Genetically Modified
- Cadherins/genetics
- Cadherins/metabolism*
- PubMed
- 20098718 Full text @ PLoS One
Citation
Mitchell, I.C., Brown, T.S., Terada, L.S., Amatruda, J.F., and Nwariaku, F.E. (2010) Effect of Vascular Cadherin Knockdown on Zebrafish Vasculature during Development. PLoS One. 5(1):e8807.
Abstract
BACKGROUND: Vascular endothelial cadherin (VE-cad) is essential for endothelial barrier integrity and vascular sprouting. However, the role of this important protein in cardiovascular development is only recently becoming apparent. METHODOLOGY/PRINCIPAL FINDINGS: To characterize the role of VE-cadherin in cardiovascular development, we analyzed cardiovascular development in a zebrafish VE-cad knockdown model. Embryos deficient in VE-cad show profoundly impaired cardiac development despite having apparently normal peripheral vasculature. Initial formation of the heart proceeds normally in knockdown embryos, but subsequent looping morphogenesis is impaired. Consistent with these results, VE-cad knockdown embryos demonstrate impaired cardiac function and early circulatory arrest. Histologic examination of knockdown embryos shows persistent, abnormal separation of the endocardial and myocardial layers. Using transmission electron microscopy, we demonstrate that endocardial junctions form poorly in VE-cad knockdown embryos, with resulting leak across the endothelial layer and reduction in the density of the cardiac jelly. CONCLUSIONS: Our results demonstrate a significant role for VE-cadherin in cardiac development independent of its effects on the formation of the peripheral vasculature.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping