PUBLICATION

In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio

Authors
Babaryka, A., Kuhn, E., and Koster, R.W.
ID
ZDB-PUB-100112-4
Date
2009
Source
Journal of Fish Biology   74(2): 452-457 (Other)
Registered Authors
Babaryka, Andreas, Köster, Reinhard W., Kühn, Enrico
Keywords
Gal4, I-SceI, meganuclease, transgenesis, zebrafish
MeSH Terms
none
PubMed
none Full text @ J. Fish Biol.
Abstract
The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping