PUBLICATION
In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio
- Authors
- Babaryka, A., Kuhn, E., and Koster, R.W.
- ID
- ZDB-PUB-100112-4
- Date
- 2009
- Source
- Journal of Fish Biology 74(2): 452-457 (Other)
- Registered Authors
- Babaryka, Andreas, Köster, Reinhard W., Kühn, Enrico
- Keywords
- Gal4, I-SceI, meganuclease, transgenesis, zebrafish
- MeSH Terms
- none
- PubMed
- none Full text @ J. Fish Biol.
Citation
Babaryka, A., Kuhn, E., and Koster, R.W. (2009) In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio. Journal of Fish Biology. 74(2):452-457.
Abstract
The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping