ZFIN ID: ZDB-PUB-100112-4
In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio
Babaryka, A., Kuhn, E., and Koster, R.W.
Date: 2009
Source: Journal of Fish Biology   74(2): 452-457 (Other)
Registered Authors: Babaryka, Andreas, Köster, Reinhard W., Kühn, Enrico
Keywords: Gal4, I-SceI, meganuclease, transgenesis, zebrafish
MeSH Terms: none
PubMed: none Full text @ J. Fish Biol.
The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.