PUBLICATION

Zebrafish cardiac enhancer trap lines: New tools for in vivo studies of cardiovascular development and disease

Authors
Poon, K.L., Liebling, M., Kondrychyn, I., Garcia-Lecea, M., and Korzh, V.
ID
ZDB-PUB-100112-31
Date
2010
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   239(3): 914-926 (Journal)
Registered Authors
Garcia-Lecea, Marta, Kondrychyn, Igor, Korzh, Vladimir, Liebling, Michael, Poon, Kar Lai
Keywords
epicardium, myocardium, endocardium, bulbus arteriosus, ventricle, atrium, valves, fast scanning confocal imaging, image rendering, fhla
MeSH Terms
  • Green Fluorescent Proteins/metabolism
  • Transgenes
  • Heart/embryology*
  • Zebrafish
  • Cardiovascular System
  • Pericardium/pathology
  • Enhancer Elements, Genetic
  • Animals
  • Heart Atria/pathology
  • Disease Models, Animal
  • Genetic Techniques*
  • Cardiovascular Diseases/pathology
  • Myocardium/metabolism*
  • Microscopy, Confocal/methods
  • Endocardium/pathology
(all 15)
PubMed
20063419 Full text @ Dev. Dyn.
Abstract
Using the transposon-mediated enhancer trap (ET), we generated 18 cardiac enhancer trap (CET) transgenic zebrafish lines. They exhibit EGFP expression in defined cell types-the endocardium, myocardium, and epicardium-or in anatomical regions of the heart-the atrium, ventricle, valves, or bulbus arteriosus. Most of these expression domains are maintained into adulthood. The genomic locations of the transposon insertions were determined by thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR). The expression pattern of EGFP in some CETs is unique and recapitulates expression of genes flanking the transposon insertion site. The CETs enabled us to capture the dynamics of the embryonic heart beating in vivo using fast scanning confocal microscopy coupled with image reconstruction, producing three-dimensional movies in time (4D) illustrating region-specific features of heart contraction. This collection of CET lines represents a toolbox of markers for in vivo studies of heart development, physiology, and drug screening.
Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
f1TgTransgenic Insertion
    smo_unspecified
      		Unspecified
      sqet27EtTransgenic Insertion
        sqet30AEtTransgenic Insertion
          sqet31EtTransgenic Insertion
            sqet33mi3AEtTransgenic Insertion
              sqet33mi32BEtTransgenic Insertion
                sqet33mi36BEtTransgenic Insertion
                  sqet33mi60AEtTransgenic Insertion
                    sqet33mi74AEtTransgenic Insertion
                      1 - 10 of 20
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      Fish
                      smounspecified/unspecified; sqet27Et
                      sqet27Et
                      sqet30AEt
                      sqet31Et
                      sqet33mi3AEt
                      sqet33mi32BEt
                      sqet33mi36BEt
                      sqet33mi60AEt
                      sqet33mi74AEt
                      sqet33mi75CEt
                      1 - 10 of 19
                      Show
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      GFPEFGGFP
                      1 - 2 of 2
                      Show
                      Mapping
                      No data available
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                      Citation
                      Poon, K.L., Liebling, M., Kondrychyn, I., Garcia-Lecea, M., and Korzh, V. (2010) Zebrafish cardiac enhancer trap lines: New tools for in vivo studies of cardiovascular development and disease. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(3):914-926.