PUBLICATION
Medaka Oct4 is expressed during early embryo development, and in primordial germ cells and adult gonads
- Authors
- Sánchez-Sánchez, A.V., Camp, E., García-España, A., Leal-Tassias, A., and Mullor, JL.
- ID
- ZDB-PUB-100105-14
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(2): 672-679 (Journal)
- Registered Authors
- Camp, Esther
- Keywords
- Oct4, medaka, PGC, gonads, pluripotency
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Biological Evolution
- Brain/metabolism
- Embryonic Development*
- Female
- Germ Cells/metabolism
- Male
- Models, Animal
- Molecular Sequence Data
- Octamer Transcription Factor-3/metabolism*
- Oryzias/embryology*
- Oryzias/metabolism
- Ovary/metabolism*
- Sequence Alignment
- Sequence Homology, Nucleic Acid
- Testis/metabolism*
- PubMed
- 20034054 Full text @ Dev. Dyn.
Citation
Sánchez-Sánchez, A.V., Camp, E., García-España, A., Leal-Tassias, A., and Mullor, JL. (2010) Medaka Oct4 is expressed during early embryo development, and in primordial germ cells and adult gonads. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(2):672-679.
Abstract
Oct4 is a crucial transcription factor for controlling pluripotency in embryonic stem cells and the epiblast of mouse embryos. We have characterized the expression pattern of medaka (Oryzias latipes) Ol-Oct4 during embryonic development and in the adult gonads. Genomic analysis showed that Ol-Oct4 is the ortholog of zebrafish spg/pou2. However, their expression patterns are not the same, suggesting that Oct4 may play different roles in zebrafish and medaka. Using specific antibodies for the Ol-Oct4 protein, we showed that Ol-Oct4 is also expressed in primordial germ cells, in the spermatogonia (male germ stem cells), and during different stages of oocyte development. These results suggest that Ol-Oct4 plays a post-embryonic role in the maturing gonads and gametes. The Ol-Oct4 mRNA and protein expression patterns are similar to those of mammalian Oct4 and introduce medaka fish as a valid model for the functional and evolutionary study of pluripotency genes in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping