PUBLICATION

Developmentally Regulated Impediments to Skin Reinnervation by Injured Peripheral Sensory Axon Terminals

Authors
O'Brien, G.S., Martin, S.M., Söllner, C., Wright, G.J., Becker, C.G., Portera-Cailliau, C., and Sagasti, A.
ID
ZDB-PUB-091215-44
Date
2009
Source
Current biology : CB   19(24): 2086-2090 (Journal)
Registered Authors
Becker, Catherina G., O'Brien, Georgeann, Sagasti, Alvaro, Söllner, Christian, Wright, Gavin J.
Keywords
MOLNEURO
MeSH Terms
  • Animals
  • Axotomy
  • DNA Primers/genetics
  • DNA, Complementary/genetics
  • Microscopy, Confocal
  • Mutagenesis, Site-Directed
  • Nerve Regeneration/physiology*
  • Neuronal Plasticity/physiology*
  • Presynaptic Terminals/physiology*
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism
  • Sensory Receptor Cells/physiology*
  • Skin/innervation*
  • Trigeminal Nerve/cytology*
  • Trigeminal Nerve Injuries
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • rhoA GTP-Binding Protein/metabolism
PubMed
19962310 Full text @ Curr. Biol.
Abstract
The structural plasticity of neurites in the central nervous system (CNS) diminishes dramatically after initial development, but the peripheral nervous system (PNS) retains substantial plasticity into adulthood. Nevertheless, functional reinnervation by injured peripheral sensory neurons is often incomplete [1-6]. To investigate the developmental control of skin reinnervation, we imaged the regeneration of trigeminal sensory axon terminals in live zebrafish larvae following laser axotomy. When axons were injured during early stages of outgrowth, regenerating and uninjured axons grew into denervated skin and competed with one another for territory. At later stages, after the establishment of peripheral arbor territories, the ability of uninjured neighbors to sprout diminished severely, and although injured axons reinitiated growth, they were repelled by denervated skin. Regenerating axons were repelled specifically by their former territories, suggesting that local inhibitory factors persist in these regions. Antagonizing the function of several members of the Nogo receptor (NgR)/RhoA pathway improved the capacity of injured axons to grow into denervated skin. Thus, as in the CNS, impediments to reinnervation in the PNS arise after initial establishment of axon arbor structure.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
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Mapping