PUBLICATION

Visualization of myelination in GFP-transgenic zebrafish

Authors
Jung, S.H., Kim, S., Chung, A.Y., Kim, H.T., So, J.H., Ryu, J., Park, H.C., and Kim, C.H.
ID
ZDB-PUB-091120-62
Date
2010
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   239(2): 592-597 (Journal)
Registered Authors
Jung, Seung-Hyun, Kim, Cheol-Hee, So, Ju-Hoon
Keywords
oligodendrocytes, myelination, transgenic zebrafish, mbp
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/metabolism*
  • Promoter Regions, Genetic
  • Schwann Cells/metabolism*
  • Animals, Genetically Modified
  • Zebrafish
  • Myelin Basic Protein/metabolism*
  • Fluorescent Dyes/metabolism
  • Oligodendroglia/metabolism*
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Embryonic Development
  • Spinal Cord/growth & development
(all 13)
PubMed
19918882 Full text @ Dev. Dyn.
Abstract
The insulation of axons in the vertebrate nervous system by myelin is essential for efficient axonal conduction. Myelination disruption and remyelination failure can cause human diseases, such as multiple sclerosis and hereditary myelin diseases. However, despite progress in understanding myelination regulation, many important questions remain unanswered. To investigate the mechanisms underlying myelination in vivo, we generated transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) under the control of the mbp promoter. This transgenic fish displayed faithful EGFP expression in oligodendrocytes and Schwann cells in embryonic and adult zebrafish. Interestingly, although myelination progressed continuously in the postembryonic central nervous system, some of the spinal cord regions were filled with unmyelinated axons even in the adult spinal cord, suggesting functional differences between myelinated and unmyelinated axons. Our results suggest that this transgenic zebrafish could be a valuable animal model to study oligodendrocyte differentiation and myelination in vivo.
Genes / Markers
Figures
Figure Gallery (4 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ck1TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    ck1Tg
    1 - 1 of 1
    Show
    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    1 - 1 of 1
    Show
    Mapping
    No data available
    Your Input Welcome
    We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
    Citation
    Jung, S.H., Kim, S., Chung, A.Y., Kim, H.T., So, J.H., Ryu, J., Park, H.C., and Kim, C.H. (2010) Visualization of myelination in GFP-transgenic zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(2):592-597.