PUBLICATION
Detection of single quantum dots in model organisms with sheet illumination microscopy
- Authors
- Friedrich, M., Nozadze, R., Gan, Q., Zelman-Femiak, M., Ermolayev, V., Wagner, T., and Harms, G.S.
- ID
- ZDB-PUB-091023-48
- Date
- 2009
- Source
- Biochemical and Biophysical Research Communications 390(3): 722-727 (Journal)
- Registered Authors
- Wagner, Toni
- Keywords
- Single-molecule tracking, Model organisms, Quantum dots, Sheet illumination microscopy, Development, Selective plane illumination microscopy
- MeSH Terms
-
- Animals
- Drosophila melanogaster/chemistry
- Drosophila melanogaster/metabolism
- Embryo, Nonmammalian/chemistry
- Embryo, Nonmammalian/metabolism
- Larva/chemistry
- Larva/metabolism
- Lighting
- Microscopy/methods
- Molecular Imaging/methods*
- Quantum Dots*
- Zebrafish/embryology
- Zebrafish/metabolism
- PubMed
- 19833091 Full text @ Biochem. Biophys. Res. Commun.
Citation
Friedrich, M., Nozadze, R., Gan, Q., Zelman-Femiak, M., Ermolayev, V., Wagner, T., and Harms, G.S. (2009) Detection of single quantum dots in model organisms with sheet illumination microscopy. Biochemical and Biophysical Research Communications. 390(3):722-727.
Abstract
Single-molecule detection and tracking is important for observing the biomolecule interactions in the microenvironment. Here we report selective plane illumination microscopy (SPIM) with single-molecule detection in living organisms, which enables fast imaging and single-molecule tracking and optical penetration beyond 300mum. We detected single nanocrystals in Drosophila larvae and zebrafish embryo. We also report our first tracking of single quantum dots during zebrafish development, which displays a transition from flow to confined motion prior to the blastula stage. The new SPIM setup represents a new technique, which enables fast single-molecule imaging and tracking in living systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping