PUBLICATION
Comparative expression profiling reveals an essential role for Raldh2 in epimorphic regeneration
- Authors
- Mathew, L.K., Sengupta, S., Franzosa, J., Perry, J., La Du, J., Andreasen, E.A., and Tanguay, R.L.
- ID
- ZDB-PUB-091023-20
- Date
- 2009
- Source
- The Journal of biological chemistry 284(48): 33642-33653 (Journal)
- Registered Authors
- Andreasen, Eric A., La Du, Jane K., Mathew, Lijoy K., Tanguay, Robyn L.
- Keywords
- CELL/Cell-Cell Interaction, CELL/Differentiation, DEVELOPMENT DIFFERENTIATION, DEVELOPMENT DIFFERENTIATION/Tissue, DEVELOPMENT DIFFERENTIATION/ Organ, GENE/Regulation, SIGNAL TRANSDUCTION
- Datasets
- GEO:GSE10188
- MeSH Terms
-
- Regeneration/drug effects
- Regeneration/genetics
- Wound Healing/drug effects
- Wound Healing/genetics
- Gene Expression Regulation, Developmental*
- In Situ Hybridization
- Oligonucleotide Array Sequence Analysis
- Retinal Dehydrogenase/genetics*
- Wnt Proteins/metabolism
- Zebrafish Proteins/genetics*
- Animals
- Signal Transduction/drug effects
- Butadienes/pharmacology
- Cluster Analysis
- Pyrroles/pharmacology
- Mitogen-Activated Protein Kinase 3/metabolism
- Female
- Nitriles/pharmacology
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/growth & development
- Gene Expression Profiling*
- Receptor, Fibroblast Growth Factor, Type 1/antagonists & inhibitors
- Receptor, Fibroblast Growth Factor, Type 1/metabolism
- Male
- Reverse Transcriptase Polymerase Chain Reaction
- Mitogen-Activated Protein Kinase 1/metabolism
- Extremities/embryology
- Extremities/growth & development
- Extremities/physiology
- Larva/genetics
- Larva/growth & development
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/injuries
- Embryo, Nonmammalian/metabolism
- PubMed
- 19801676 Full text @ J. Biol. Chem.
Citation
Mathew, L.K., Sengupta, S., Franzosa, J., Perry, J., La Du, J., Andreasen, E.A., and Tanguay, R.L. (2009) Comparative expression profiling reveals an essential role for Raldh2 in epimorphic regeneration. The Journal of biological chemistry. 284(48):33642-33653.
Abstract
Zebrafish have the remarkable ability to regenerate body parts including the heart and fins by a process referred to as epimorphic regeneration. Recent studies have illustrated that similar to adult zebrafish, early life stage-larvae also possess the ability to regenerate the caudal fin. A comparative microarray analysis was used to determine the degree of conservation in gene expression among the regenerating adult caudal fin, adult heart and larval fin. Results indicate that these tissues respond to amputation/injury with strikingly similar genomic responses. Comparative analysis revealed raldh2, a rate-limiting enzyme for the synthesis of Retinoic acid (RA), as one of the most highly induced genes across the three regeneration platforms. In situ localization and functional studies indicate that raldh2 expression is critical for the formation of wound epithelium and blastema. Patterning during regenerative outgrowth was considered to be the primary function of RA signaling; however our results suggest that it is also required for early stages of tissue regeneration. Expression of raldh2 is regulated by Wnt and Fgf/ERK signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping