PUBLICATION
Ultraviolet Radiation Dose to be Applied in Recipient Zebrafish Embryos for Germ-line Chimaerism is Strain Dependent
- Authors
- Francisco-Simão, M., Cardona-Costa, J., Perez Camps, M., and García-Ximénez, F.
- ID
- ZDB-PUB-091005-3
- Date
- 2010
- Source
- Reproduction in domestic animals = Zuchthygiene 45(6): 1098-1103 (Journal)
- Registered Authors
- Cardona-Costa, Jose
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo Culture Techniques
- Embryo, Nonmammalian/radiation effects*
- Germ Cells/physiology*
- Germ Cells/radiation effects
- Radiation Chimera/physiology*
- Zebrafish/classification
- Zebrafish/genetics*
- PubMed
- 19788522 Full text @ Reprod. Domest. Anim.
Citation
Francisco-Simão, M., Cardona-Costa, J., Perez Camps, M., and García-Ximénez, F. (2010) Ultraviolet Radiation Dose to be Applied in Recipient Zebrafish Embryos for Germ-line Chimaerism is Strain Dependent. Reproduction in domestic animals = Zuchthygiene. 45(6):1098-1103.
Abstract
Contents Germ-line chimaerism is a powerful technique that has proved to be useful to produce viable gametes when transplanted blastomeres colonize the germinal ridges in recipient embryos and obtaining offspring from such transplanted cells. In fish, ionizing radiations were commonly used for embryo penalization to cancelling the cell participation of recipient embryos in development and in gamete production. The ultraviolet (UV) radiation when compared with other radiation types is cheaper, easier and no special installations are required for its use. So, the aim of this work was to establish the optimal UV radiation dose to be applied in zebrafish embryos at mid-blastula transition stage of development, in order to use them as penalized recipient embryos in futures chimaerism assays. A UV germicide lamp was used as radiation source (0.529 mW/cm(2)). Four exposure levels and three exposure times of UV radiation were tested. The survival rates obtained with the non-dechorionated embryos without lid group suggested that it could be the optimal exposure level to achieve the objective proposed. With the obtained results, we concluded that this UV radiation dose for 60 and 30 s are optimal parameters to penalize recipient wild and gold strain zebrafish embryos, respectively in chimaerism assays, but without involving their survival and apparently normal development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping