PUBLICATION
Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic development
- Authors
- Zhou, H., and Clapham, D.E.
- ID
- ZDB-PUB-090914-11
- Date
- 2009
- Source
- Proceedings of the National Academy of Sciences of the United States of America 106(37): 15750-15755 (Journal)
- Registered Authors
- Keywords
- ALR1, transporter, TRPM, zebrafish, KMG104-AM
- MeSH Terms
-
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- PubMed
- 19717468 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Magnesium (Mg(2+)) is the second most abundant cation in cells, yet relatively few mechanisms have been identified that regulate cellular levels of this ion. The most clearly identified Mg(2+) transporters are in bacteria and yeast. Here, we use a yeast complementary screen to identify two mammalian genes, MagT1 and TUSC3, as major mechanisms of Mg(2+) influx. MagT1 is universally expressed in all human tissues and its expression level is up-regulated in low extracellular Mg(2+). Knockdown of either MagT1 or TUSC3 protein significantly lowers the total and free intracellular Mg(2+) concentrations in mammalian cell lines. Morpholino knockdown of MagT1 and TUSC3 protein expression in zebrafish embryos results in early developmental arrest; excess Mg(2+) or supplementation with mammalian mRNAs can rescue the effects. We conclude that MagT1 and TUSC3 are indispensable members of the vertebrate plasma membrane Mg(2+) transport system.
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