PUBLICATION
Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic development
- Authors
- Zhou, H., and Clapham, D.E.
- ID
- ZDB-PUB-090914-11
- Date
- 2009
- Source
- Proceedings of the National Academy of Sciences of the United States of America 106(37): 15750-15755 (Journal)
- Registered Authors
- Keywords
- ALR1, transporter, TRPM, zebrafish, KMG104-AM
- MeSH Terms
-
- Animals, Genetically Modified
- Pregnancy
- Protein Structure, Secondary
- Membrane Proteins/antagonists & inhibitors
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Oligodeoxyribonucleotides, Antisense/genetics
- Tissue Distribution
- Male
- Saccharomyces cerevisiae/genetics
- Saccharomyces cerevisiae/growth & development
- Saccharomyces cerevisiae/metabolism
- Female
- Embryonic Development/genetics
- Embryonic Development/physiology*
- Animals
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Cation Transport Proteins/antagonists & inhibitors
- Cation Transport Proteins/chemistry
- Cation Transport Proteins/genetics
- Cation Transport Proteins/metabolism*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Base Sequence
- Genetic Complementation Test
- Tumor Suppressor Proteins/antagonists & inhibitors
- Tumor Suppressor Proteins/genetics
- Tumor Suppressor Proteins/metabolism*
- Jurkat Cells
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Ion Transport
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Humans
- Cell Line
- Saccharomyces cerevisiae Proteins/genetics
- Saccharomyces cerevisiae Proteins/metabolism
- Magnesium/metabolism*
- PubMed
- 19717468 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Zhou, H., and Clapham, D.E. (2009) Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic development. Proceedings of the National Academy of Sciences of the United States of America. 106(37):15750-15755.
Abstract
Magnesium (Mg(2+)) is the second most abundant cation in cells, yet relatively few mechanisms have been identified that regulate cellular levels of this ion. The most clearly identified Mg(2+) transporters are in bacteria and yeast. Here, we use a yeast complementary screen to identify two mammalian genes, MagT1 and TUSC3, as major mechanisms of Mg(2+) influx. MagT1 is universally expressed in all human tissues and its expression level is up-regulated in low extracellular Mg(2+). Knockdown of either MagT1 or TUSC3 protein significantly lowers the total and free intracellular Mg(2+) concentrations in mammalian cell lines. Morpholino knockdown of MagT1 and TUSC3 protein expression in zebrafish embryos results in early developmental arrest; excess Mg(2+) or supplementation with mammalian mRNAs can rescue the effects. We conclude that MagT1 and TUSC3 are indispensable members of the vertebrate plasma membrane Mg(2+) transport system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping