PUBLICATION
Generating chimeric zebrafish embryos by transplantation
- Authors
- Kemp, H.A., Carmany-Rampey, A., and Moens, C.
- ID
- ZDB-PUB-090727-6
- Date
- 2009
- Source
- Journal of visualized experiments : JoVE (29): (Journal)
- Registered Authors
- Carmany-Rampey, Amanda, Kemp, Hilary, Moens, Cecilia
- Keywords
- none
- MeSH Terms
-
- Animals
- Blastula/transplantation
- Cell Transplantation/methods*
- Embryo Transfer
- Embryo, Nonmammalian/cytology
- Female
- Gastrula/transplantation
- Transplantation Chimera/embryology*
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 19617875 Full text @ J. Vis. Exp.
Citation
Kemp, H.A., Carmany-Rampey, A., and Moens, C. (2009) Generating chimeric zebrafish embryos by transplantation. Journal of visualized experiments : JoVE. (29).
Abstract
One of the most powerful tools used to gain insight into complex developmental processes is the analysis of chimeric embryos. A chimera is defined as an organism that contains cells from more than one animal; mosaics are one type of chimera in which cells from more than one genotype are mixed, usually wild-type and mutant. In the zebrafish, chimeras can be readily made by transplantation of cells from a donor embryo into a host embryo at the appropriate embryonic stage. Labeled donor cells are generated by injection of a lineage marker, such as a fluorescent dye, into the one-cell stage embryo. Labeled donor cells are removed from donor embryos and introduced into unlabeled host embryos using an oil-controlled glass pipette mounted on either a compound or dissecting microscope. Donor cells can in some cases be targeted to a specific region or tissue of the developing blastula or gastrula stage host embryo by choosing a transplantation site in the host embryo based on well-established fate maps.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping