PUBLICATION
The synthetic substance hypoxanthine 3-N-oxide elicits alarm reactions in zebrafish (Danio rerio)
- Authors
- Parra, K.V., Adrian, J.C. Jr, and Gerlai, R.
- ID
- ZDB-PUB-090716-4
- Date
- 2009
- Source
- Behavioural brain research 205(2): 336-341 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- Alarm substance, Anxiety, Fear, H3NO, Zebrafish
- MeSH Terms
-
- Analysis of Variance
- Animals
- Fear/physiology*
- Female
- Freezing Reaction, Cataleptic/physiology
- Hypoxanthines/chemistry
- Hypoxanthines/metabolism*
- Male
- Motor Activity/physiology
- Random Allocation
- Zebrafish
- PubMed
- 19583985 Full text @ Behav. Brain Res.
Citation
Parra, K.V., Adrian, J.C. Jr, and Gerlai, R. (2009) The synthetic substance hypoxanthine 3-N-oxide elicits alarm reactions in zebrafish (Danio rerio). Behavioural brain research. 205(2):336-341.
Abstract
Zebrafish, one of the preferred study species of geneticists, is gaining increasing popularity in behavioral neuroscience. This small and prolific species may be an excellent tool with which the biological mechanisms of vertebrate brain function and behavior are investigated. Zebrafish has been proposed as a model organism in the analysis of fear responses and human anxiety disorders. Species-specific cues signaling the presence of predators have been successfully utilized in such research. Zebrafish has been shown to respond to its natural alarm substance with species-typical fear reactions. However, the extraction of this alarm substance and ascertaining its consistent dosing has been problematic. A synthetic substance with a known chemical identity and molecular weight would allow precise dosing and experimental control. Previously, the chemical component, hypoxanthine 3-N-oxide, common to several fish alarm substances has been identified and has been shown to elicit alarm reactions in fish species belonging to the Osteriophysan superorder. In the current study we investigate the effect of hypoxanthine 3-N-oxide by exposing zebrafish to three different concentrations of this synthetic substance. Our results show that the substance efficaciously induces species-typical fear reactions increasing the number of erratic movement episodes and jumps in zebrafish. We discuss the translational relevance of our findings and conclude that hypoxanthine 3-N-oxide will have utility to elicit fear responses in the laboratory in a precisely controlled manner in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping