PUBLICATION

Expression of five frizzleds during zebrafish craniofacial development

Authors

Sisson, B.E., and Topczewski, J.

ID

ZDB-PUB-090716-22

Date

2009

Source

Gene expression patterns : GEP 9(7): 520-527 (Journal)

Registered Authors

Sisson, Barbara E., Topczewski, Jacek

Keywords

Zebrafish, frizzled 2, frizzled 6, frizzled 7a, frizzled 7b, frizzled 8a, cartilage, craniofacial, Wnt, planar cell polarity

MeSH Terms

Zebrafish Proteins/genetics*
Zebrafish Proteins/metabolism
Gene Expression Regulation, Developmental*
Mutation
Neurogenesis
Animals
Receptors, Cell Surface/genetics*
Receptors, Cell Surface/metabolism
Frizzled Receptors/genetics
Frizzled Receptors/metabolism
Embryo, Nonmammalian/metabolism
Zebrafish/growth & development*
Receptors, G-Protein-Coupled/genetics
Receptors, G-Protein-Coupled/metabolism
Body Patterning
Wnt Proteins/genetics
Wnt Proteins/metabolism

(all 17)

PubMed

19595791 Full text @ Gene Expr. Patterns

Abstract

Wnt/Planar Cell Polarity (PCP) signaling is critical for proper animal development. While initially identified in Drosophila, this pathway is also essential for the proper development of vertebrates. Zebrafish mutants, defective in the Wnt/PCP pathway, frequently display defects in convergence and extension gastrulation movements and additional later abnormalities including problems with craniofacial cartilage morphogenesis. Although multiple Frizzled (Fzd) homologues, Wnt receptors, were identified in zebrafish, it is unknown which Fzd plays a role in shaping the early larvae head skeleton. In an effort to determine which Frizzleds are involved in this process, we analyzed the expression of five zebrafish frizzled homologues fzd2, 6, 7a, 7b, and 8a from 2 - 4 days post fertilization (dpf). During the analyzed developmental time points fzd2 and fzd6 are broadly expressed throughout the head, while the expression of fzd7a, 7b and 8a is much more restricted. Closer examination revealed that fzd7b is expressed in the neural crest and the mesodermal core of the pharyngeal arches and in the chondrocytes of newly stacked craniofacial cartilage elements. However, fzd7a is only expressed in the neural crest of the pharyngeal arches and fzd8a is expressed in the pharyngeal endoderm.

Genes / Markers

Probes

Probe	Type	All Figures
	CDNA	Show all Figures
	CDNA	Show all Figures
	CDNA	Show all Figures
	CDNA	Show all Figures

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
gpc4^m818/m818	standard conditions	Protruding-mouth	convergent extension involved in axis elongation disrupted, abnormal	Fig. S1
gpc4^m818/m818	standard conditions	Protruding-mouth	convergent extension involved in axis elongation disrupted, abnormal	Fig. 5
gpc4^m818/m818	standard conditions	Protruding-mouth	whole organism decreased length, abnormal	Fig. S1
gpc4^m818/m818	standard conditions	Protruding-mouth	whole organism decreased length, abnormal	Fig. 5
wnt11f2^tz216/tz216	standard conditions	Protruding-mouth	convergent extension involved in axis elongation disrupted, abnormal	Fig. S1
wnt11f2^tz216/tz216	standard conditions	Protruding-mouth	convergent extension involved in axis elongation disrupted, abnormal	Fig. 5
wnt11f2^tz216/tz216	standard conditions	Protruding-mouth	eye fused with eye, abnormal	Fig. S1
wnt11f2^tz216/tz216	standard conditions	Protruding-mouth	eye fused with eye, abnormal	Fig. 5
wnt11f2^tz216/tz216	standard conditions	Protruding-mouth	whole organism decreased length, abnormal	Fig. S1
wnt11f2^tz216/tz216	standard conditions	Protruding-mouth	whole organism decreased length, abnormal	Fig. 5

1 - 10 of 14

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
m818		Point Mutation	gpc4
ta98		Point Mutation	wnt5b
tz216		Point Mutation	wnt11f2
y1Tg	Tg(fli1:EGFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
AB
gpc4^m818/m818
wnt5b^ta98/ta98
wnt11f2^tz216/tz216
WT
y1Tg/+ (AB)

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Sisson et al., 2009 ZDB-PUB-090716-22 PMID:19595791

Expression of five frizzleds during zebrafish craniofacial development

Sisson et al., 2009

ZDB-PUB-090716-22
PMID:19595791