PUBLICATION
Identification and expression analysis of two zebrafish E2F5 genes during oogenesis and development
- Authors
- Yang, Q., Hu, J., Ye, D., Zhao, C., Song, S., Gong, W., Tan, Z., and Song, P.
- ID
- ZDB-PUB-090714-9
- Date
- 2010
- Source
- Molecular biology reports 37(4): 1773-1780 (Journal)
- Registered Authors
- Song, Ping, Ye, Ding
- Keywords
- E2F5, Oocyte development, Zebrafish, Ovary, Lens
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- E2F5 Transcription Factor/chemistry
- E2F5 Transcription Factor/genetics*
- E2F5 Transcription Factor/metabolism
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Exons/genetics
- Female
- Gene Expression Profiling*
- Gene Expression Regulation, Developmental*
- Introns/genetics
- Molecular Sequence Data
- Oocytes/cytology
- Oocytes/metabolism
- Oogenesis/genetics*
- Ovary/cytology
- Ovary/metabolism
- Phylogeny
- Protein Structure, Tertiary
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 19578977 Full text @ Mol. Biol. Rep.
Citation
Yang, Q., Hu, J., Ye, D., Zhao, C., Song, S., Gong, W., Tan, Z., and Song, P. (2010) Identification and expression analysis of two zebrafish E2F5 genes during oogenesis and development. Molecular biology reports. 37(4):1773-1780.
Abstract
During the process of screening genes that are preferentially expressed in zebrafish germ cells, we isolated an E2F family member zE2F5b, which is a truncated isoform of zebrafish E2F5a. The E2F transcription factors are thought to play an essential role in cell cycle progression. Reverse transcription polymerase chain reaction and whole mount in situ hybridization analyses have demonstrated that both zE2F5a and zE2F5b mRNAs are predominantly expressed in stage I and II oocytes. In early development, the two zE2F5 transcripts are expressed by many parts of the embryo and are then restricted to the brain area. Interestingly, zE2F5b mRNA is detected later in the lens and optic tectum while zE2F5a mRNA is absent. Our data suggests that E2F5 may play important roles in zebrafish brain, eye and ovarian development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping