PUBLICATION
The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly
- Authors
- Sampath, S.C., Ohi, R., Leismann, O., Salic, A., Pozniakovski, A., and Funabiki, H.
- ID
- ZDB-PUB-090706-12
- Date
- 2004
- Source
- Cell 118(2): 187-202 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence/genetics
- DNA, Complementary/analysis
- DNA, Complementary/genetics
- Xenopus Proteins/genetics
- Xenopus Proteins/isolation & purification
- PubMed
- 15260989 Full text @ Cell
Abstract
In cells lacking centrosomes, such as those found in female meiosis, chromosomes must nucleate and stabilize microtubules in order to form a bipolar spindle. Here we report the identification of Dasra A and Dasra B, two new components of the vertebrate chromosomal passenger complex containing Incenp, Survivin, and the kinase Aurora B, and demonstrate that this complex is required for chromatin-induced microtubule stabilization and spindle formation. The failure of microtubule stabilization caused by depletion of the chromosomal passenger complex was rescued by codepletion of the microtubule-depolymerizing kinesin MCAK, whose activity is negatively regulated by Aurora B. By contrast, we present evidence that the Ran-GTP pathway of chromatin-induced microtubule nucleation does not require the chromosomal passenger complex, indicating that the mechanisms of microtubule assembly by these two pathways are distinct. We propose that the chromosomal passenger complex regulates local MCAK activity to permit spindle formation via stabilization of chromatin-associated microtubules.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping