PUBLICATION

Identification of vasculature-specific genes by microarray analysis of etsrp/etv2 overexpressing zebrafish embryos

Authors
Wong, K.S., Proulx, K., Rost, M.S., and Sumanas, S.
ID
ZDB-PUB-090616-41
Date
2009
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   238(7): 1836-1850 (Journal)
Registered Authors
Sumanas, Saulius
Keywords
zebrafish, vasculogenesis, angiogenesis, vascular endothelial, microarray, etsrp, etv2, ER71, myeloid
MeSH Terms
  • Oligonucleotide Array Sequence Analysis
  • Gene Expression Profiling
  • Animals
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
  • Embryo, Nonmammalian
  • Up-Regulation
  • Blood Vessels/embryology
  • Blood Vessels/metabolism*
  • Animals, Genetically Modified
  • Organ Specificity/genetics
  • Gene Expression Regulation, Developmental
(all 13)
PubMed
19504456 Full text @ Dev. Dyn.
Abstract
Signaling pathways controlling vasculogenesis, angiogenesis, and myelopoiesis are still poorly understood, in part because not all genes important for vasculature or myeloid cell formation have been characterized. To identify novel potential regulators of vasculature and myeloid cell formation we performed microarray analysis of zebrafish embryos that overexpress Ets1-related protein (Etsrp/Etv2/ER71), sufficient to induce vasculogenesis and myelopoiesis (Sumanas and Lin [2006] Development 121:3141-3150; Lee [2008] Cell Stem Cell 2:497-507; Sumanas et al. [2008] Blood 111:4500-4510). We performed sequence homology and expression analysis for up-regulated genes that were novel or previously unassociated with the zebrafish vasculature formation. Angiotensin II type 2 receptor (agtr2), src homology 2 domain containing E (she), mannose receptor C1 (mrc1), endothelial cell-specific adhesion molecule (esam), yes-related kinase (yrk/fyn), zinc finger protein, multitype 2b (zfpm2b/fog2b), and stabilin 2 (stab2) were specifically expressed in vascular endothelial cells during early development while keratin18 expression was localized to the myeloid cells. Identification of vasculature and myeloid-specific genes will be important for dissecting molecular mechanisms of vasculogenesis/angiogenesis and myelopoiesis.
Genes / Markers
Probes
Probe Type All Figures
CDNAShow all Figures
CDNAShow all Figures
1 - 2 of 2
Show
Figures
Figure Gallery (9 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m39
    		Deficiency
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    etsrpMO1-etsrpMRPHLNO
    etsrpMO2-etsrpMRPHLNO
    1 - 2 of 2
    Show
    Fish
    Fish
    Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39 (INDO)
    WT
    WT + MO1-etsrp + MO2-etsrp
    1 - 3 of 3
    Show
    Antibodies
    No data available
    Orthology
    Gene Orthology
    agtr1a
    agtr2
    esamb
    fyna
    mrc1a
    mrc1b
    mrc2
    shda
    shdb
    she
    1 - 10 of 12
    Show
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
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    Citation
    Wong, K.S., Proulx, K., Rost, M.S., and Sumanas, S. (2009) Identification of vasculature-specific genes by microarray analysis of etsrp/etv2 overexpressing zebrafish embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(7):1836-1850.