PUBLICATION
Characterization, molecular modelling and developmental expression of zebrafish manganese superoxide dismutase
- Authors
- Lin, C.T., Tseng, W.C., Hsiao, N.W., Chang, H.H., and Ken, C.F.
- ID
- ZDB-PUB-090616-30
- Date
- 2009
- Source
- Fish & shellfish immunology 27(2): 318-324 (Journal)
- Registered Authors
- Ken, Chuian-Fu
- Keywords
- Manganese superoxide dismutase (Mn–SOD), Enzyme properties, Three dimensional homology modelling, Developmental expression, Oxidative stress
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Gene Expression Regulation, Developmental*
- Humans
- Models, Molecular*
- Molecular Sequence Data
- Oxidative Stress/physiology
- Protein Structure, Tertiary
- Sequence Alignment
- Superoxide Dismutase/chemistry*
- Superoxide Dismutase/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 19501168 Full text @ Fish Shellfish Immunol.
Citation
Lin, C.T., Tseng, W.C., Hsiao, N.W., Chang, H.H., and Ken, C.F. (2009) Characterization, molecular modelling and developmental expression of zebrafish manganese superoxide dismutase. Fish & shellfish immunology. 27(2):318-324.
Abstract
A 977bp cDNA containing an open reading frame encoding 224 amino acid residues of manganese superoxide dismutase was cloned from zebrafish (zMn-SOD). The deduced amino acid sequence showed high identity with the sequences of Mn-SODs from human (85.1%) to nematode (61.6%). The three-dimensional structure model was superimposed on the relative domains of human Mn-SOD with the root mean square (rms) deviation of 0.0919 A. The recombinant mature zMn-SOD with enzyme activity was purified using His-tag technique. The half-life of the enzyme is approximately 48min and its thermal inactivation rate constant k(d) is 0.0154min(-1)at 70 degrees C. The enzyme was active under a broad pH (2.2-11.2) and in the presence of up to 4 % SDS. Real-time RT-PCR assay was used to detect the zMn-SOD mRNA expression during the developmental stages following a challenge with paraquat. A high level expression of mn-sod mRNA was detected at the cleavage stage, but decreased significantly under paraquat treatment. The results indicated that Mn-SOD plays an important role during embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping