PUBLICATION
The origins of hematopoietic and endothelial development in zebrafish
- Authors
- Vogeli, K.
- ID
- ZDB-PUB-090521-34
- Date
- 2006
- Source
- Ph.D. Thesis : 78p (Thesis)
- Registered Authors
- Vogeli, Kevin
- Keywords
- none
- MeSH Terms
- none
- PubMed
- none
Citation
Vogeli, K. (2006) The origins of hematopoietic and endothelial development in zebrafish. Ph.D. Thesis. :78p.
Abstract
It has been thought for over 85 years that blood and blood vessels share a common developmental origin. Early experiments inferred the presence of a progenitor cell that could give rise to both blood and blood vessels called the hemangioblast. Modern genetic and cell culture experiments have provided supporting evidence; however, the presence of the bipotential hemangioblast had not been demonstrated in a developing embryo. With this in mind we constructed single-cell resolution fate maps of the early zebrafish embryo. By labeling individual cells in the zebrafish embryo and then assessing their developmental potential, we were able to demonstrate the presence of hemangioblasts. These hemangioblasts localize to a specific region of the embryo and may provide a link between primitive and definitive hematopoiesis. The first two chapters of this study are describing these findings. The last chapter of this work focuses on the mechanism of pectoral fin development in zebrafish. While these studies are more preliminary, they provided the technical groundwork for the single cell labeling experiments conducted elsewhere.
Errata / Notes
Ph.D. Thesis, University of California, San Francisco
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping