PUBLICATION
Novel and unexpected functions of zebrafish CCAAT box binding transcription factor (NF-Y) B subunit during cartilages development
- Authors
- Chen, Y.H., Lin, Y.T., and Lee, G.H.
- ID
- ZDB-PUB-090518-22
- Date
- 2009
- Source
- Bone 44(5): 777-784 (Journal)
- Registered Authors
- Chen, Yau-Hung
- Keywords
- Bone, Cartilage, Morpholino, Neural crest cell, Nuclear factor Y, Zebrafish
- MeSH Terms
-
- Animals
- Apoptosis/genetics
- Blotting, Western
- Bone and Bones/embryology
- Bone and Bones/metabolism
- CCAAT-Binding Factor/genetics
- CCAAT-Binding Factor/metabolism
- CCAAT-Binding Factor/physiology*
- Cartilage/embryology*
- Collagen Type II
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental*/genetics
- Gene Expression Regulation, Developmental*/physiology
- Head/embryology
- In Situ Hybridization
- In Situ Nick-End Labeling
- Neural Crest/enzymology
- Neural Crest/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- SOX9 Transcription Factor
- Transcription Factors/metabolism
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- PubMed
- 19442608 Full text @ Bone
Citation
Chen, Y.H., Lin, Y.T., and Lee, G.H. (2009) Novel and unexpected functions of zebrafish CCAAT box binding transcription factor (NF-Y) B subunit during cartilages development. Bone. 44(5):777-784.
Abstract
We used zebrafish as a model to study the biological functions of NF-YB during early development. Both RT-PCR and whole-mount in situ hybridization experiments revealed that nf-yb was a maternally inherited gene. Later, its expression was restricted in the future head cartilages as well as in the developing notochord. Embryos after injection with nf-yb-morpholino displayed reduced-head phenotypes, including smaller head (WT, length of head, L: 0.515+/-0.019 mm, width of head, W: 0.323+/-0.077 mm; nf-yb-morphant, L: 0.347+/-0.037 mm; W: 0.266+/-0.018 mm), sharpen Meckel's cartilage, loss of ceratobrachial, and enlarged angles of ceratohyal (WT: 72.6+/-9.4 degrees ; nf-yb-morphant: 110.0+/-32.5 degrees ). Subsequently, those abnormalities can be rescued after injection with capped nf-yb mRNA. TUNEL assay suggested that large amounts of cell apoptosis appeared in the head region of nf-yb-morphants. Staining with digoxigenin-labeled dlx2a, sox9a, runx2b and col2a1 riboprobes showed that nf-yb-morphants displayed reduced amounts of cranial neural crest cells which are required for mandibular and branchial arches formation. These observations clearly indicate that knockdown of nf-yb translation induced parts of cranial neural crest cells apoptosis, affected cartilages formation and consequently caused reduced-head phenotypes. These findings uncover a novel and unexpected role for NF-YB as a critical modulator of neural crest cell's gene expression governing embryonic cartilage growth.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping