ZFIN ID: ZDB-PUB-090505-8
Line scan fluorescence correlation spectroscopy for three-dimensional microfluidic flow velocity measurements
Pan, X., Shi, X., Korzh, V., Yu, H., and Wohland, T.
Date: 2009
Source: Journal of Biomedical Optics   14(2): 024049 (Journal)
Registered Authors: Korzh, Vladimir
Keywords: none
MeSH Terms:
  • Algorithms*
  • Image Interpretation, Computer-Assisted/methods*
  • Microfluidics/methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence/methods*
PubMed: 19405777 Full text @ J. Biomed. Opt.
The flow direction of microfluidics in biological applications is not limited to two dimensions, but often extends to three dimensions. Currently there are optical methods available for the measurement of 3-D microfluidic flow vectors, but with low spatial resolution. Line scan fluorescence correlation spectroscopy (FCS) was proposed to determine flow directions in 2-D within microchannels and small blood vessels in our previous work. Importantly, its spatial resolution was demonstrated to be as good as 0.5 mum. In this work, we extend line scan FCS to the third dimension for the characterization of 3-D flow velocity vectors. The spatial resolution is close to the diffraction limit using a scan length of 0.5 mum in all three dimensions. The feasibility of line scan FCS for 3-D microfluidic flow is verified by measurements in microchannels and small blood vessels of zebrafish embryos.