PUBLICATION
In toto imaging of embryogenesis with confocal time-lapse microscopy
- Authors
- Megason, S.G.
- ID
- ZDB-PUB-090424-12
- Date
- 2009
- Source
- Methods in molecular biology (Clifton, N.J.) 546: 317-332 (Chapter)
- Registered Authors
- Megason, Sean
- Keywords
- Confocal microscopy, Two-photon, In toto imaging, In vivo, Time lapse, Zebrafish, Image Analysis, Fluorescent, GFP, Live cell imaging
- MeSH Terms
-
- Animals
- Cell Lineage
- Cell Movement
- Embryonic Development*
- Female
- Green Fluorescent Proteins
- Image Processing, Computer-Assisted/methods
- Male
- Microscopy, Confocal/instrumentation
- Microscopy, Confocal/methods*
- Photons
- Specimen Handling/methods
- Staining and Labeling/methods
- Time Factors
- Zebrafish/embryology*
- PubMed
- 19378112 Full text @ Meth. Mol. Biol.
Citation
Megason, S.G. (2009) In toto imaging of embryogenesis with confocal time-lapse microscopy. Methods in molecular biology (Clifton, N.J.). 546:317-332.
Abstract
Microscopy has been one of the most direct and powerful tools since the beginning of biological research. Continued advances such as confocal and two-photon fluorescence microscopy and fluorescent proteins now make imaging useful at a variety of spatial scales (molecules, circuits, cells, tissues, and even whole embryos) and temporal scales (
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping