|ZFIN ID: ZDB-PUB-090422-22|
Production of pseudotyped retrovirus and the generation of proviral transgenic zebrafish
Jao, L.E., and Burgess, S.M.
|Source:||Methods in molecular biology (Clifton, N.J.) 546: 13-30 (Chapter)|
|Registered Authors:||Burgess, Shawn|
|Keywords:||Zebrafish, Retrovirus, Pseudotyped Moloney murine leukemia virus, Insertional mutagenesis, Injection|
|PubMed:||19378095 Full text @ Meth. Mol. Biol.|
Jao, L.E., and Burgess, S.M. (2009) Production of pseudotyped retrovirus and the generation of proviral transgenic zebrafish. Methods in molecular biology (Clifton, N.J.). 546:13-30.
ABSTRACTThis chapter describes a method for generation of the high-titer pseudotyped Moloney murine leukemia virus (MLV) that efficiently infects zebrafish embryos (i.e., more than 25 retroviral copies per cell). Injection techniques are also described for production of the retrovirus-infected mosaic "founder" fish. We describe a quantitative PCR (qPCR)-based assay as a quick way to assess the infectivity after each round of viral production and injection. Most of the required equipment is commercially available and commonly present in most research laboratories.
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