PUBLICATION

Effect of Gametes Aging on Their Activation and Fertilizability in Zebrafish (Danio rerio)

Authors
Cardona-Costa, J., Pérez-Camps, M., García-Ximénez, F., and Espinós, F.J.
ID
ZDB-PUB-090422-18
Date
2009
Source
Zebrafish   6(1): 93-95 (Journal)
Registered Authors
Cardona-Costa, Jose
Keywords
none
MeSH Terms
  • Animals
  • Cellular Senescence*
  • Female
  • Fertilization
  • Male
  • Oocytes/cytology*
  • Spermatozoa/cytology*
  • Zebrafish*
PubMed
19374552 Full text @ Zebrafish
Abstract
The zebrafish represents an important model organism for biological research. In this context, in vitro collection and fertilization of zebrafish gametes are basic and widely used techniques for many topical research works. In this work, the fertilization ability and normal embryo development of gold-type zebrafish sperm and eggs were re-evaluated after being stored for different times at 8 degrees C in a modified medium (Hanks' saline supplemented with 1.5 g BSA and 0.1 g ClNa; 320 mOsm, pH 7.4). Results obtained indicated that the temporal limits usually recommended for zebrafish sperm to fertilize fresh eggs (2 h) could be extended for up to 24 h without significant differences compared with fresh sperm. In contrast, the rapid egg aging observed (even less than 1 h) recommends minimizing as far as possible the egg storage time before fertilization. These results suggest a possible strain difference in the fertilization response.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping