ZFIN ID: ZDB-PUB-090330-4
NF-kappaB and Snail1a coordinate the cell cycle with gastrulation
Liu, X., Huang, S., Ma, J., Li, C., Zhang, Y., and Luo, L.
Date: 2009
Source: The Journal of cell biology   184(6): 805-815 (Journal)
Registered Authors: Luo, Lingfei, Ma, Jun
Keywords: none
MeSH Terms:
  • Animals
  • Apoptosis
  • Blastula/metabolism*
  • Cell Cycle*/genetics
  • Cell Cycle Proteins/metabolism
  • Cell Movement
  • Cell Nucleus/metabolism
  • Cytoplasm/metabolism
  • Gastrula/metabolism*
  • Gastrulation*/genetics
  • Gene Expression Regulation, Developmental
  • Nervous System/embryology
  • Nervous System/metabolism
  • Promoter Regions, Genetic
  • RNA, Messenger/metabolism
  • Signal Transduction*/genetics
  • Transcription Factor RelA/genetics
  • Transcription Factor RelA/metabolism*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Transcriptional Activation
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 19307597 Full text @ J. Cell Biol.
The cell cycle needs to strictly coordinate with developmental processes to ensure correct generation of the body plan and different tissues. However, the molecular mechanism underlying the coordination remains largely unknown. In this study, we investigate how the cell cycle coordinates gastrulation cell movements in zebrafish. We present a system to modulate the cell cycle in early zebrafish embryos by manipulating the geminin-Cdt1 balance. Alterations of the cell cycle change the apoptotic level during gastrulation, which correlates with the nuclear level of antiapoptotic nuclear factor kappaB (NF-kappaB). NF-kappaB associates with the Snail1a promoter region on the chromatin and directly activates Snail1a, an important factor controlling cell delamination, which is the initial step of mesendodermal cell movements during gastrulation. In effect, the cell cycle coordinates the delamination of mesendodermal cells through the transcription of Snail1a. Our results suggest a molecular mechanism by which NF-kappaB and Snail1a coordinate the cell cycle through gastrulation.