PUBLICATION
Sodium benzoate exposure downregulates the expression of tyrosine hydroxylase and dopamine transporter in dopaminergic neuronsin developing zebrafish
- Authors
- Chen, Q., Huang, N.N., Huang, J.T., Chen, S., Fan, J., Li, C., and Xie, F.K.
- ID
- ZDB-PUB-090319-21
- Date
- 2009
- Source
- Birth defects research. Part B, Developmental and reproductive toxicology 86(2): 85-91 (Journal)
- Registered Authors
- Keywords
- Sodium benzoate, Zebrafish embryo, Dopaminergic neurons, Neurotoxicity
- MeSH Terms
-
- Animals
- Base Sequence
- DNA, Complementary/genetics
- Diencephalon/drug effects*
- Diencephalon/embryology
- Diencephalon/metabolism
- Dopamine Plasma Membrane Transport Proteins/biosynthesis*
- Dopamine Plasma Membrane Transport Proteins/genetics
- Dose-Response Relationship, Drug
- Down-Regulation/drug effects
- Enzyme Induction/drug effects
- Food Preservatives/toxicity*
- In Situ Hybridization
- Larva/drug effects
- Microscopy, Fluorescence
- Molecular Sequence Data
- Neurons/drug effects*
- Neurons/metabolism
- Random Allocation
- Sodium Benzoate/toxicity*
- Swimming
- Tyrosine 3-Monooxygenase/biosynthesis*
- Tyrosine 3-Monooxygenase/genetics
- Zebrafish/embryology*
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics
- PubMed
- 19294673 Full text @ Birth Defects Res. B Dev. Reprod. Toxicol.
Citation
Chen, Q., Huang, N.N., Huang, J.T., Chen, S., Fan, J., Li, C., and Xie, F.K. (2009) Sodium benzoate exposure downregulates the expression of tyrosine hydroxylase and dopamine transporter in dopaminergic neuronsin developing zebrafish. Birth defects research. Part B, Developmental and reproductive toxicology. 86(2):85-91.
Abstract
BACKGROUND: Recent data have demonstrated that treatment with sodium benzoate (SB) leads to significant developmental defects in motor neuron axons and neuromuscular junctions in zebrafish larvae, thereby implying that SB can be neurotoxic. This study examined whether SB affects the development of dopaminergic neurons in the zebrafish brain. METHODS: Zebrafish embryos were exposed to different concentrations of SB for various durations, during which the survival rates were recorded, the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) in the neurons in the ventral diencephalon were detected by in situ hybridization and immunofluorescence, and the locomotor activity of larval zebrafish was measured. RESULTS: The survival rates were significantly decreased with the increase of duration and dose of SB-treatment. Compared to untreated clutch mates (untreated controls), treatment with SB significantly downregulated expression of TH and DAT in neurons in the ventral diencephalon of 3-day post-fertilization (dpf) zebrafish embryos in a dose-dependent manner. Furthermore, there was a marked decrease in locomotor activity in zebrafish larvae at 6dpf in response to SB treatment. CONCLUSIONS: The results suggest that SB exposure can cause significantly decreased survival rates of zebrafish embryos in a time- and dose-dependent manner and downregulated expression of TH and DAT in dopaminergic neurons in the zebrafish ventral diencephalon, which results in decreased locomotor activity of zebrafish larvae. This study may provide some important information for further elucidating the mechanism underlying SB-induced developmental neurotoxicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping