PUBLICATION

Definitive hematopoietic stem/progenitor cells manifest distinct differentiation output in the zebrafish VDA and PBI

Authors
Jin, H., Sood, R., Xu, J., Zhen, F., English, M.A., Liu, P.P., and Wen, Z.
ID
ZDB-PUB-090204-17
Date
2009
Source
Development (Cambridge, England)   136(4): 647-654 (Journal)
Registered Authors
English, Milton A., Liu, Pu Paul, Sood, Raman, Wen, Zilong
Keywords
Zebrafish, Hematopoiesis, Ventral wall of dorsal aorta (VDA), Posterior blood island (PBI), Lineage differentiation, Ontogeny
MeSH Terms
  • Animal Structures/cytology*
  • Animal Structures/embryology*
  • Animals
  • Aorta/cytology*
  • Aorta/embryology*
  • Cell Differentiation*
  • Cell Lineage
  • Cell Movement
  • Embryo, Nonmammalian/cytology
  • Erythrocytes/cytology
  • Erythroid Cells/cytology
  • Erythropoiesis
  • Fertilization
  • Hematopoietic Stem Cells/cytology*
  • Myeloid Cells/cytology
  • Zebrafish/embryology*
PubMed
19168679 Full text @ Development
Abstract
One unique feature of vertebrate definitive hematopoiesis is the ontogenic switching of hematopoietic stem cells from one anatomical compartment or niche to another. In mice, hematopoietic stem cells are believed to originate in the aorta-gonad-mesonephros (AGM), subsequently migrate to the fetal liver (FL) and finally colonize the bone marrow (BM). Yet, the differentiation potential of hematopoietic stem cells within early niches such as the AGM and FL remains incompletely defined. Here, we present in vivo analysis to delineate the differentiation potential of definitive hematopoietic stem/progenitor cells (HSPCs) in the zebrafish AGM and FL analogies, namely the ventral wall of dorsal aorta (VDA) and the posterior blood island (PBI), respectively. Cell fate mapping and analysis of zebrafish runx1(w84x) and vlad tepes (vlt(m651)) mutants revealed that HSPCs in the PBI gave rise to both erythroid and myeloid lineages. However, we surprisingly found that HSPCs in the VDA were not quiescent but were uniquely adapted to generate myeloid but not erythroid lineage cells. We further showed that such distinct differentiation output of HSPCs was, at least in part, ascribed to the different micro-environments present in these two niches. Our results highlight the importance of niche in shaping the differentiation output of developing HSPCs.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes