ZFIN ID: ZDB-PUB-090116-11
Fluorescence-activated cell sorting (FACS) of whole mount in situ hybridization (WISH) labelled haematopoietic cell populations in the zebrafish
Dobson, J.T., Da'as, S., McBride, E.R., and Berman, J.N.
Date: 2009
Source: British journal of haematology   144(5): 732-735 (Journal)
Registered Authors: Berman, Jason
Keywords: zebrafish, FACS, haematopoiesis, flow cytometry, morphology
MeSH Terms:
  • Animals
  • Female
  • Flow Cytometry/methods
  • Hematopoietic Stem Cells/cytology*
  • In Situ Hybridization/methods
  • Models, Animal
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 19133983 Full text @ Br. J. Haematol.
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ABSTRACT
The zebrafish is a robust animal model for studying vertebrate haematopoiesis and immune cell interactions. However, fluorescence activated cell sorting (FACS) has been limited due to a paucity of available functional zebrafish antibodies. We have developed a technique combining FACS with whole mount in situ hybridization (WISH) that enables the sorting and examining of fixed zebrafish blood cell populations at different stages of embryonic development, providing the opportunity to correlate RNA expression data with cellular morphology.
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